为中国西北部一种濒危物种开发微卫星标记。
Development of SSR markers for , a vulnerable species from northwestern China.
作者信息
Wu Mingying, Shi Xiaojun, Tan Dunyan
机构信息
Xinjiang Key Laboratory of Grassland Resources and Ecology and Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology College of Grassland and Environment Sciences Xinjiang Agricultural University Ürümqi 830052 People's Republic of China.
College of Biology and Environmental Sciences Jishou University Jishou 416000 People's Republic of China.
出版信息
Appl Plant Sci. 2019 Oct 24;7(10):e11297. doi: 10.1002/aps3.11297. eCollection 2019 Oct.
PREMISE
(Fabaceae) is a vulnerable species found in the cold deserts of northwestern China. We aimed to characterize polymorphic microsatellite loci for to support future studies of population genetic dynamics and conservation management of the species.
METHODS AND RESULTS
We used next-generation sequencing to detect polymorphic microsatellites. Twenty-five potential microsatellite loci were identified, 12 of which were polymorphic and present in the three study populations of . Levels of observed and expected heterozygosities were 0.000-1.000 and 0.000-0.827, respectively. Furthermore, two and five of the 12 developed primers were successfully amplified in two congeneric species, and , respectively.
CONCLUSIONS
These newly developed microsatellite markers can be used to determine population diversity and to develop conservation strategies in .
前提
（豆科）是一种在中国西北寒冷沙漠中发现的易危物种。我们旨在鉴定该物种的多态微卫星位点，以支持未来对其种群遗传动态和保护管理的研究。
方法与结果
我们使用下一代测序来检测多态微卫星。共鉴定出25个潜在的微卫星位点，其中12个具有多态性且存在于该物种的三个研究种群中。观察到的杂合度水平和预期杂合度水平分别为0.000 - 1.000和0.000 - 0.827。此外，12个开发的引物中分别有2个和5个在两个同属物种和中成功扩增。
结论
这些新开发的微卫星标记可用于确定该物种的种群多样性并制定保护策略。
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