Wang Yong, Zhao Liang, Huo Yazhen, Zhou Feng, Wu Wei, Lu Feng, Yang Xue, Guo Xiaoxuan, Chen Peng, Deng Qianchun, Ji Baoping
Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Engineering, China Agricultural University, Beijing 100083, China.
Nutrients. 2016 May 2;8(5):245. doi: 10.3390/nu8050245.
Dietary proanthocyanidins (PACs) as health-protective agents have become an important area of human nutrition research because of their potent bioactivities. We investigated the retinoprotective effects of PACs from sea buckthorn (Hippophae rhamnoides L.) seed against visible light-induced retinal degeneration in vivo. Pigmented rabbits were orally administered sea buckthorn seed PACs (50 and 100 mg/kg/day) for 14 consecutive days of pre-illumination and seven consecutive days of post-illumination. Retinal function was quantified via electroretinography 7 days after light exposure. Retinal damage was evaluated by measuring the thickness of the full-thickness retina and outer nuclear layer 7 days after light exposure. Sea buckthorn seed PACs significantly attenuated the destruction of electroretinograms and maintained the retinal structure. Increased retinal photooxidative damage was expressed by the depletion of glutathione peroxidase and catalase activities, the decrease of total antioxidant capacity level and the increase of malondialdehyde level. Light exposure induced a significant increase of inflammatory cytokines (IL-1β, TNF-α and IL-6) and angiogenesis (VEGF) levels in retina. Light exposure upregulated the expression of pro-apoptotic proteins Bax and caspase-3 and downregulated the expression of anti-apoptotic protein Bcl-2. However, sea buckthorn seed PACs ameliorated these changes induced by light exposure. Sea buckthorn seed PACs mediated the protective effect against light-induced retinal degeneration via antioxidant, anti-inflammatory and antiapoptotic mechanisms.
由于具有强大的生物活性，膳食原花青素（PACs）作为健康保护剂已成为人类营养研究的一个重要领域。我们研究了沙棘（沙棘属）种子中的PACs对可见光诱导的体内视网膜变性的视网膜保护作用。在光照前连续14天和光照后连续7天，给色素沉着的兔子口服沙棘种子PACs（50和100毫克/千克/天）。在光照7天后通过视网膜电图对视网膜功能进行定量。在光照7天后通过测量全层视网膜和外核层的厚度来评估视网膜损伤。沙棘种子PACs显著减轻了视网膜电图的破坏并维持了视网膜结构。视网膜光氧化损伤增加表现为谷胱甘肽过氧化物酶和过氧化氢酶活性的消耗、总抗氧化能力水平的降低以及丙二醛水平的升高。光照诱导视网膜中炎性细胞因子（IL-1β、TNF-α和IL-6）和血管生成（VEGF）水平显著增加。光照上调促凋亡蛋白Bax和caspase-3的表达并下调抗凋亡蛋白Bcl-2的表达。然而，沙棘种子PACs改善了光照诱导的这些变化。沙棘种子PACs通过抗氧化、抗炎和抗凋亡机制介导对光诱导的视网膜变性的保护作用。
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