Shekhar Shubhendu, Agrawal Lalit, Mishra Divya, Buragohain Alak Kumar, Unnikrishnan Mullath, Mohan Chokkappan, Chakraborty Subhra, Chakraborty Niranjan
National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
Sci Rep. 2016 May 5;6:25384. doi: 10.1038/srep25384.
Storage proteins in plants, because of high nutrient value, have been a subject of intensive investigation. These proteins are synthesized de novo in the cytoplasm and transported to the storage organelles where they serve as reservoir of energy and supplement of nitrogen during rapid growth and development. Sweetpotato is the seventh most important food crop worldwide, and has a significant contribution to the source of nutrition, albeit with low protein content. To determine the behaviour of seed storage proteins in non-native system, a seed albumin, AmA1, was overexpressed in sweetpotato with an additional aim of improving nutritional quality of tuber proteins. Introduction of AmA1 imparted an increase in protein and amino acid contents as well as the phytophenols. The proteometabolomics analysis revealed a rebalancing of the proteome, with no significant effects on the global metabolome profile of the transgenic tubers. Additionally, the slower degradation of starch and cellulose in transgenic tubers, led to increased post-harvest durability. Present study provides a new insight into the role of a seed storage protein in the modulation of photoassimilate movement and nutrient acquisition.
由于具有较高的营养价值，植物中的贮藏蛋白一直是深入研究的对象。这些蛋白质在细胞质中重新合成，并被运输到贮藏细胞器中，在植物快速生长和发育过程中，它们作为能量储备和氮源补充。甘薯是全球第七大重要粮食作物，尽管蛋白质含量低，但对营养来源有重要贡献。为了确定种子贮藏蛋白在非天然系统中的行为，一种种子清蛋白AmA1在甘薯中过表达，另外一个目的是提高块茎蛋白的营养质量。AmA1的导入使蛋白质、氨基酸含量以及植物酚类物质增加。蛋白质代谢组学分析显示蛋白质组重新平衡，对转基因块茎的整体代谢组谱没有显著影响。此外，转基因块茎中淀粉和纤维素降解较慢，导致收获后耐久性增强。本研究为种子贮藏蛋白在调节光合产物运输和养分获取中的作用提供了新的见解。
