Wang Lin, Lim Chee Kent, Dang Hongyue, Hanson Thomas E, Klotz Martin G
Evolutionary and Genomic Microbiology, Department of Biological Sciences, University of North Carolina at Charlotte Charlotte, NC, USA.
State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Sciences, Xiamen UniversityXiamen, China; Joint Research Center for Carbon Sink: The Institute of Marine Microbes and Ecospheres, Xiamen University and the Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of SciencesQingdao, China.
Front Microbiol. 2016 Apr 14;7:512. doi: 10.3389/fmicb.2016.00512. eCollection 2016.
An ammonia-oxidizing bacterium, strain D1FHS, was enriched into pure culture from a sediment sample retrieved in Jiaozhou Bay, a hyper-eutrophic semi-closed water body hosting the metropolitan area of Qingdao, China. Based on initial 16S rRNA gene sequence analysis, strain D1FHS was classified in the genus Nitrosococcus, family Chromatiaceae, order Chromatiales, class Gammaproteobacteria; the 16S rRNA gene sequence with highest level of identity to that of D1FHS was obtained from Nitrosococcus halophilus Nc4(T). The average nucleotide identity between the genomes of strain D1FHS and N. halophilus strain Nc4 is 89.5%. Known species in the genus Nitrosococcus are obligate aerobic chemolithotrophic ammonia-oxidizing bacteria adapted to and restricted to marine environments. The optimum growth (maximum nitrite production) conditions for D1FHS in a minimal salts medium are: 50 mM ammonium and 700 mM NaCl at pH of 7.5 to 8.0 and at 37°C in dark. Because pertinent conditions for other studied Nitrosococcus spp. are 100-200 mM ammonium and <700 mM NaCl at pH of 7.5 to 8.0 and at 28-32°C, D1FHS is physiologically distinct from other Nitrosococcus spp. in terms of substrate, salt, and thermal tolerance.
从中国青岛大都市区的超富营养半封闭水体胶州湾采集的沉积物样本中富集培养出一株氨氧化细菌，菌株D1FHS。基于最初的16S rRNA基因序列分析，菌株D1FHS被归类于γ-变形菌纲、着色菌目、着色菌科、亚硝化球菌属；与D1FHS的16S rRNA基因序列具有最高同源性的序列来自嗜盐亚硝化球菌Nc4(T)。菌株D1FHS和嗜盐亚硝化球菌菌株Nc4的基因组之间的平均核苷酸同源性为89.5%。亚硝化球菌属中的已知物种是专性需氧化能自养氨氧化细菌，适应并局限于海洋环境。在基本盐培养基中，D1FHS的最佳生长（最大亚硝酸盐产生）条件为：50 mM铵和700 mM氯化钠，pH为7.5至8.0，37°C，黑暗条件。由于其他已研究的亚硝化球菌属物种的相关条件为100 - 200 mM铵和<700 mM氯化钠，pH为7.5至8.0，28 - 32°C，因此D1FHS在底物、盐和热耐受性方面在生理上与其他亚硝化球菌属物种不同。
