Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Int J Syst Evol Microbiol. 2016 Aug;66(8):3022-3028. doi: 10.1099/ijsem.0.001139. Epub 2016 May 6.
A novel dissimilatory Fe(III)-reducing bacterium, designated strain GSS09T, was isolated from a compost sample by using a solid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS09T were anaerobic, Gram-stain-positive, motile, endospore-forming and rod-shaped. Growth occurred at 30-55 °C (optimum 50 °C), at pH 6.5-9.0 (optimum pH 7.5) and in the presence of 0-3 % (w/v) NaCl (optimum 1 %). Both sulfur compounds such as sulfate, sulfite and thiosulfate and Fe(III) oxides such as ferrihydrite could be utilized as electron acceptors. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSS09T was related closely to Desulfotomaculum hydrothermale Lam5T (94.5 % sequence similarity). The major fatty acids were C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The G+C content of the genomic DNA was 49.1 mol%. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS09T is considered to represent a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum ferrireducens sp. nov. is proposed. The type strain is GSS09T (=KCTC 15523T=MCCC 1K01254T).
从含有醋酸盐和水铁矿分别作为电子供体和电子受体的固体培养基中，分离到一株新型异化还原三价铁的细菌，命名为 GSS09T 株。GSS09T 菌株细胞为厌氧菌，革兰氏阳性，可运动，芽孢内生，呈杆状。该菌株在 30-55°C（最佳 50°C）、pH6.5-9.0（最佳 pH7.5）和 0-3%（w/v）NaCl（最佳 1%）的条件下生长。硫酸盐、亚硫酸盐和硫代硫酸盐等含硫化合物和水铁矿等三价铁氧化物都可以作为电子受体被利用。基于 16S rRNA 基因序列的系统发育分析表明，GSS09T 株与嗜热脱硫梭菌 Lam5T（94.5%序列相似性）密切相关。主要脂肪酸为 C16:0 和 C16:1ω7c/C16:1ω6c。基因组 DNA 的 G+C 含量为 49.1mol%。基于系统发育分析、表型特征和生理测试，GSS09T 株被认为代表脱硫梭菌属的一个新种，因此命名为脱硫梭菌 ferrireducens 新种。模式菌株为 GSS09T（=KCTC 15523T=MCCC 1K01254T）。
