Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Nanyang Normal University, Henan, China.
College of Life Sciences and Agricultural Engineering, Nanyang Normal University, Nanyang, Henan, China.
Plant Signal Behav. 2022 Dec 31;17(1):2058256. doi: 10.1080/15592324.2022.2058256.
To study the allelopathic effect of the extracts of , the changes of cell density of were measured. The anti-algae allelopathic effect of different organic solvent extracts of was evaluated, and the physiological, biochemical indexes were determined to discuss the mechanism of algal inhibition. The results showed that the petroleum ether, dichloromethane and ethyl acetate extracts showed various inhibitory effects on . Among them, ethyl acetate extract was the most strongly allelopathic part with the semi-effect concentration(EC) of 59.6 mg L, the central polarity part of inhibitory activity. The contents of chlorophyll a(Chl a) and phycobiliproteins(PBPs) of were decreased under the concentration of 200 mg L ethyl acetate extract, which indicated that the photosynthesis of was inhibited. The consent of microcystins was lower compared to control under 200 mg L. The contents of superoxide dismutase(SOD), malondialdehyde(MDA) and hydrogen peroxide(HO) of cell pellets were firstly increased and then decreased, which suggested that the algal cells were seriously damaged by oxidation. The results indicated that the extracts of had inhibitory effect on , and the ethyl acetate extract was the central part of the inhibitory substances, which affected photosynthesis and caused peroxidation damage to inhibit cell proliferation. These findings will be helpful for exploration and application of allelopathic effects of in harmful algae control.
为了研究 的提取物的化感作用，测量了 的细胞密度变化。评价了 不同有机溶剂提取物的抗藻化感效应，并测定了生理生化指标，以探讨抑藻机制。结果表明，石油醚、二氯甲烷和乙酸乙酯提取物对 表现出不同的抑制作用。其中，乙酸乙酯提取物是最强烈的化感部分，半效浓度（EC）为 59.6mg/L，是抑制活性的中心极性部分。在 200mg/L 乙酸乙酯提取物浓度下， 的叶绿素 a（Chl a）和藻蓝蛋白（PBPs）含量降低，表明 的光合作用受到抑制。与对照相比，200mg/L 时微囊藻毒素的含量较低。细胞沉淀中超氧化物歧化酶（SOD）、丙二醛（MDA）和过氧化氢（HO）的含量先增加后减少，表明藻细胞受到严重氧化损伤。结果表明， 提取物对 有抑制作用，乙酸乙酯提取物是抑制物质的中心部分，它影响光合作用，并通过过氧化损伤抑制细胞增殖。这些发现将有助于探索和应用 在有害藻类控制中的化感效应。
