来自西班牙拉科鲁尼亚省布尔戈湾贻贝中的人札幌病毒
Human Sapovirus in Mussels from Ría do Burgo, A Coruña (Spain).
作者信息
Varela Miguel F, Hooper Annalise S, Rivadulla Enrique, Romalde Jesús L
机构信息
Departamento de Microbioloxía e Parasitoloxía, CIBUS-Facultade de Bioloxía, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
School of Marine Sciences, University of Portsmouth, Portsmouth, UK.
出版信息
Food Environ Virol. 2016 Sep;8(3):187-93. doi: 10.1007/s12560-016-9242-8. Epub 2016 May 7.
The prevalence and genetical diversity of human Sapovirus were studied during an 18-month study in Ría do Burgo, an estuary nearby the city of A Coruña in Galicia (NW Spain). Sapovirus was detected using RT-qPCR procedure in 30 out of 80 mussel samples (37.5 %). Quantifications ranged from 2.2 × 10(3) to 2.1 × 10(5) RNA copies per gram of digestive tissue. Detection occurred mainly during the cold and rainy seasons of the period studied. Sequences obtained could be distributed into 5 genotypes being the most abundant GI.1 and GI.3. Results obtained indicate that the hydrodynamic characteristics of the harvesting area and the proximity of population density clearly influence the presence of the virus in shellfish.
在西班牙西北部加利西亚自治区拉科鲁尼亚市附近的一个河口布尔戈河进行的为期18个月的研究中，对人札幌病毒的流行情况和遗传多样性进行了研究。使用RT-qPCR方法在80个贻贝样本中的30个（37.5%）中检测到了札幌病毒。每克消化组织的定量范围为2.2×10³至2.1×10⁵个RNA拷贝。检测主要发生在研究期间的寒冷和多雨季节。获得的序列可分为5种基因型，其中最丰富的是GI.1和GI.3。获得的结果表明，捕捞区域的水动力特征和人口密度的接近程度明显影响贝类中病毒的存在。
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