K R Vinod, Jones Denny, Udupa Venkatesha
Department of Toxicology, Glenmark Research Centre, Navi Mumbai 400709, India.
MethodsX. 2016 Apr 8;3:315-9. doi: 10.1016/j.mex.2016.04.001. eCollection 2016.
In this paper, we describe an additional step to the standard method of heat induced antigen retrieval to improve the detection of antibody staining of formalin fixed paraffin embedded tissue sections. Direct heating of tissues in buffer is an efficient epitope retrieval method but often results in the damage or loss of tissues. In this modified method, before keeping in buffer for heating, we overlapped the tissue on the slide with a plain slide by clipping one end using a normal paperclip, keeping a minimum gap between the slides. Tissues heated in this way in buffer had following advantages over normal heat treatment for epitope retrieval. •Tissues were intact even at high temperatures which improved the quality of staining by preventing fold, damage or detachment of tissues from the slides.•The method is very safe and economical compared to the methods using microwave or pressure cooker.•This simple method also appears to be very effective and less time consuming compared to the existing methods.
在本文中，我们描述了对热诱导抗原修复标准方法的一个额外步骤，以改进福尔马林固定石蜡包埋组织切片抗体染色的检测。在缓冲液中直接加热组织是一种有效的抗原表位修复方法，但常常导致组织受损或丢失。在这种改良方法中，在放入缓冲液加热之前，我们用一个普通回形针夹住一端，将载玻片上的组织与一张空白载玻片重叠，使玻片之间保持最小间隙。与常规热处理进行抗原表位修复相比，用这种方法在缓冲液中加热的组织具有以下优点。•即使在高温下组织也保持完整，通过防止组织折叠、损伤或从玻片上脱落，提高了染色质量。•与使用微波炉或高压锅的方法相比，该方法非常安全且经济。•与现有方法相比，这种简单方法似乎也非常有效且耗时更少。
