微孔阵列揭示了转化人类细胞克隆扩增过程中的细胞异质性。
Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells.
作者信息
Chang Tim C, Tang Weiliang, Koh William Jen Hoe, Rettie Alexander J E, Emond Mary J, Monnat Raymond J, Folch Albert
机构信息
Departments of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Departments of Pathology, University of Washington, Seattle, WA 98195, USA.
出版信息
Technology (Singap World Sci). 2015 Dec;3(4):163-171. doi: 10.1142/S2339547815200046.
Abstract
We developed micromolded microwell arrays to study the proliferation and senescence of single cells. Microwell arrays were designed to be compatible with conventional cell culture protocols to simplify cell loading, cell culture, and imaging. We demonstrated the utility of these arrays by measuring the proliferation and senescence of isogenic cells which expressed or had been depleted of the human Werner syndrome protein. Our results allowed us to reveal cell-to-cell heterogeneity in proliferation in WRN+ and WRN-depleted fibroblasts during clonal growth.
摘要
我们开发了微模塑微孔阵列来研究单细胞的增殖和衰老。微孔阵列的设计与传统细胞培养方案兼容，以简化细胞接种、细胞培养和成像。我们通过测量表达或已缺失人类沃纳综合征蛋白的同基因细胞的增殖和衰老，证明了这些阵列的实用性。我们的结果使我们能够揭示克隆生长过程中WRN +和WRN缺失的成纤维细胞增殖的细胞间异质性。
相似文献
1
Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells.微孔阵列揭示了转化人类细胞克隆扩增过程中的细胞异质性。
Technology (Singap World Sci). 2015 Dec;3(4):163-171. doi: 10.1142/S2339547815200046.
2
Functional role of the Werner syndrome RecQ helicase in human fibroblasts.Werner综合征RecQ解旋酶在人成纤维细胞中的功能作用。
Aging Cell. 2007 Feb;6(1):53-61. doi: 10.1111/j.1474-9726.2006.00260.x.
3
Clonal expansion under the microscope: studying lymphocyte activation and differentiation using live-cell imaging.显微镜下的克隆扩增：利用活细胞成像技术研究淋巴细胞的激活与分化
Immunol Cell Biol. 2016 Mar;94(3):242-9. doi: 10.1038/icb.2015.104. Epub 2015 Dec 22.
4
WRN, the protein deficient in Werner syndrome, plays a critical structural role in optimizing DNA repair.WRN蛋白，即沃纳综合征中缺乏的蛋白质，在优化DNA修复过程中发挥着关键的结构作用。
Aging Cell. 2003 Aug;2(4):191-9. doi: 10.1046/j.1474-9728.2003.00052.x.
5
Telomerase expression prevents replicative senescence but does not fully reset mRNA expression patterns in Werner syndrome cell strains.端粒酶表达可防止复制性衰老，但不能完全重置沃纳综合征细胞株中的mRNA表达模式。
FASEB J. 2001 Apr;15(6):1014-20. doi: 10.1096/fj.00-0104com.
6
Estrogen prevents senescence through induction of WRN, Werner syndrome protein.雌激素通过诱导 WRN（ Werner 综合征蛋白）来防止衰老。
Horm Res Paediatr. 2010;74(1):33-40. doi: 10.1159/000313366. Epub 2010 Apr 15.
7
Werner syndrome protein limits MYC-induced cellular senescence.沃纳综合征蛋白限制MYC诱导的细胞衰老。
Genes Dev. 2003 Jul 1;17(13):1569-74. doi: 10.1101/gad.1100303.
8
9
3D hydrogel-based microwell arrays as a tumor microenvironment model to study breast cancer growth.基于3D水凝胶的微孔阵列作为研究乳腺癌生长的肿瘤微环境模型。
Biomed Mater. 2017 Mar 15;12(2):025009. doi: 10.1088/1748-605X/aa5d5c.
10
Poly(N-isopropylacrylamide)-coated microwell arrays for construction and recovery of multicellular spheroids.用于构建和回收多细胞球体的聚（N-异丙基丙烯酰胺）涂层的微孔阵列。
J Biosci Bioeng. 2013 Jun;115(6):695-9. doi: 10.1016/j.jbiosc.2012.12.017. Epub 2013 Jan 21.
引用本文的文献
1
Rapid laser ablation-based fabrication of high-density polymer microwell arrays for high-throughput cellular studies.基于快速激光烧蚀的高密度聚合物微孔阵列制备用于高通量细胞研究。
Lab Chip. 2025 Mar 25;25(7):1813-1822. doi: 10.1039/d4lc01058b.
2
A high throughput array microhabitat platform reveals how light and nitrogen colimit the growth of algal cells.高通量阵列微生境平台揭示了光和氮如何共同限制藻类细胞的生长。
Sci Rep. 2024 Apr 29;14(1):9860. doi: 10.1038/s41598-024-59041-3.
3
New tools for immunologists: models of lymph node function from cells to tissues.免疫学家的新工具：从细胞到组织的淋巴结功能模型。
Front Immunol. 2023 May 10;14:1183286. doi: 10.3389/fimmu.2023.1183286. eCollection 2023.
4
Cellular microarrays for assessing single-cell phenotypic changes in vascular cell populations.用于评估血管细胞群体中单细胞表型变化的细胞微阵列。
Biomed Microdevices. 2023 Mar 16;25(2):11. doi: 10.1007/s10544-023-00651-5.
5
Fusing MEMS technology with lab-on-chip: nanoliter-scale silicon microcavity arrays for digital DNA quantification and multiplex testing.将微机电系统技术与芯片实验室相结合：用于数字DNA定量和多重检测的纳升规模硅微腔阵列
Microsyst Nanoeng. 2020 Oct 5;6:82. doi: 10.1038/s41378-020-00187-1. eCollection 2020.
本文引用的文献
1
Cancer heterogeneity: implications for targeted therapeutics.癌症异质性：对靶向治疗的影响。
Br J Cancer. 2013 Feb 19;108(3):479-85. doi: 10.1038/bjc.2012.581. Epub 2013 Jan 8.
2
Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling.WRN 和 BLM 这两种 RECQ 解旋酶在人类细胞中具有不同的功能，分别参与了受羟基脲诱导而停滞的复制叉的恢复和延伸。
DNA Repair (Amst). 2013 Feb 1;12(2):128-39. doi: 10.1016/j.dnarep.2012.11.005. Epub 2012 Dec 17.
3
Aging, cellular senescence, and cancer.衰老、细胞衰老和癌症。
Annu Rev Physiol. 2013;75:685-705. doi: 10.1146/annurev-physiol-030212-183653. Epub 2012 Nov 8.
4
Fractional proliferation: a method to deconvolve cell population dynamics from single-cell data.分数增殖：一种从单细胞数据中推断细胞群体动态的方法。
Nat Methods. 2012 Sep;9(9):923-8. doi: 10.1038/nmeth.2138. Epub 2012 Aug 12.
5
High-throughput stem-cell niches.
Nat Methods. 2011 Oct 28;8(11):915-6. doi: 10.1038/nmeth.1745.
6
Simple room temperature bonding of thermoplastics and poly(dimethylsiloxane).热塑性塑料和聚二甲基硅氧烷的简单室温键合。
Lab Chip. 2011 Mar 7;11(5):962-5. doi: 10.1039/c0lc00272k. Epub 2010 Dec 8.
7
Human RECQ helicases: roles in DNA metabolism, mutagenesis and cancer biology.人类 RECQ 解旋酶：在 DNA 代谢、诱变和癌症生物学中的作用。
Semin Cancer Biol. 2010 Oct;20(5):329-39. doi: 10.1016/j.semcancer.2010.10.002. Epub 2010 Oct 8.
8
The human WRN and BLM RecQ helicases differentially regulate cell proliferation and survival after chemotherapeutic DNA damage.人类 WRN 和 BLM RecQ 解旋酶在化疗性 DNA 损伤后对细胞增殖和存活的调控存在差异。
Cancer Res. 2010 Aug 15;70(16):6548-55. doi: 10.1158/0008-5472.CAN-10-0475. Epub 2010 Jul 27.
9
Large-scale investigation of the olfactory receptor space using a microfluidic microwell array.利用微流控微井阵列对嗅觉受体空间进行大规模研究。
Lab Chip. 2010 May 7;10(9):1120-7. doi: 10.1039/b920585c. Epub 2010 Feb 10.
10
Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application.鉴定和避免 siRNA 脱靶效应，以进行靶标鉴定和治疗应用。
Nat Rev Drug Discov. 2010 Jan;9(1):57-67. doi: 10.1038/nrd3010.
Zotero 插件