Akan M, Ozbilgin S, Boztas N, Celik A, Ozkardesler S, Ergur B U, Guneli E, Sisman A R, Akokay P, Meseri R
Department of Anesthesiology and Reanimation, Faculty of Medicine, Dokuz Eylül University, Izmir, Turkey.
Eur Rev Med Pharmacol Sci. 2016 Apr;20(8):1642-55.
Ischemia/reperfusion (I/R) injury is a major cause of acute organ dysfunction and I/R related acute renal failure is a common clinical problem. Diabetes mellitus is defined as a risk factor for the development of acute renal injury as diabetic nephropathy compromises the renal tolerance to ischemia. The aim of this study was to investigate the protective effect of magnesium sulfate in a diabetic rat renal I/R injury model.
Diabetes mellitus was induced using streptozotocin. Thirty-five rats were divided into five groups: Group I: Nondiabetic sham group; Group II: Diabetic sham group; Group III: Diabetic I/R group; Group IV: Diabetic I/R + prophylactic (preischemic) MgSO4; and Group V: Diabetic I/R + therapeutic (following reperfusion) MgSO4 group. MgSO4 was administered 200 mg/kg intraperitoneally. Renal I/R (45 min ischemia + 4 h reperfusion) was induced in both kidneys. Histomorphological, immunohistochemical (caspase-3 and iNOS) and biochemical (BUN, Creatinine) methods were performed to assess the blood and tissue samples.
Histomorphological injury scores and immunostaining intensities (for both caspase-3 and iNOS) were significantly lower in the MgSO4 administered groups (prophylactic and therapeutic) than in the Diabetic IR group. There were no significant differences in biochemical parameters (BUN, Cr) between the MgSO4 administered groups and the Diabetic IR group.
In the present study, it was demonstrated by histomorphological and immunohistochemical methods that magnesium sulfate administration before ischemia or following reperfusion significantly reduced renal I/R injury in a diabetic rat model.
缺血/再灌注（I/R）损伤是急性器官功能障碍的主要原因，与I/R相关的急性肾衰竭是常见的临床问题。糖尿病被定义为急性肾损伤发生的一个危险因素，因为糖尿病肾病会损害肾脏对缺血的耐受性。本研究的目的是探讨硫酸镁在糖尿病大鼠肾I/R损伤模型中的保护作用。
采用链脲佐菌素诱导糖尿病。35只大鼠分为五组：第一组：非糖尿病假手术组；第二组：糖尿病假手术组；第三组：糖尿病I/R组；第四组：糖尿病I/R+预防性（缺血前）硫酸镁组；第五组：糖尿病I/R+治疗性（再灌注后）硫酸镁组。硫酸镁以200mg/kg腹腔注射给药。对双侧肾脏进行肾I/R（45分钟缺血+4小时再灌注）。采用组织形态学、免疫组织化学（半胱天冬酶-3和诱导型一氧化氮合酶）和生化（血尿素氮、肌酐）方法对血液和组织样本进行评估。
硫酸镁给药组（预防性和治疗性）的组织形态学损伤评分和免疫染色强度（半胱天冬酶-3和诱导型一氧化氮合酶）均显著低于糖尿病I/R组。硫酸镁给药组与糖尿病I/R组之间的生化参数（血尿素氮、肌酐）无显著差异。
在本研究中，通过组织形态学和免疫组织化学方法证明，在缺血前或再灌注后给予硫酸镁可显著减轻糖尿病大鼠模型中的肾I/R损伤。
