七氟醚对肾缺血再灌注糖尿病大鼠 NF-кB 和 TNF-α 表达的影响。
Effects of sevoflurane on NF-кB and TNF-α expression in renal ischemia-reperfusion diabetic rats.
机构信息
Department of Endocrinology and Metabolism, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
Department of Dermatology, Dongguan Maternal and Child Health Hospital, Dongguan, China.
出版信息
Inflamm Res. 2017 Oct;66(10):901-910. doi: 10.1007/s00011-017-1071-1. Epub 2017 Jul 6.
BACKGROUND
Ischemia-reperfusion (I/R) injury is the main reason of acute renal failure. However, inflammatory response and cell apoptosis are important mechanisms implicated in I/R injury. Recent studies indicated that nuclear factor kappa B (NF-кB) and tumor necrosis factor α (TNF-α) are both involved in these mechanisms. Sevoflurane reduces NF-кB and TNF-α expression in rats' heart and decreases their renal I/R injury. However, few studies are available regarding the effect of sevoflurane on kidney of diabetic rats. Therefore, the aim of this study was to evaluate sevoflurane effect on NF-кB and TNF-α expression in diabetic rats to decrease renal I/R injury.
METHODS
Male Sprague-Dawley rats were divided into five groups: Group A, non-diabetic rats underwent sham operation; Group B, non-diabetic rats with renal I/R injury; Group C, diabetic rats underwent sham operation; Group D, diabetic rats with renal I/R injury; Group E, diabetic rats with renal I/R injury after sevoflurane pretreatment. Rats of Group E were exposed to 2.5% sevoflurane for 30 min. After 24 h, creatinine (Cr), blood urea nitrogen (BUN), renal cell apoptosis, and NF-кB and TNF-α expression in kidney were assessed.
RESULTS
Renal cell apoptosis, NF-кB, and TNF-α expression were significantly higher in diabetic rats with renal I/R injury group compared to diabetic rats that underwent sham operation (P < 0.01). These changes were significantly reduced by sevoflurane (P < 0.01).
CONCLUSION
Sevoflurane exerted a protective effect against renal injury by lowering the expression of NF-кB and TNF-α in renal I/R diabetic rats.
背景
缺血再灌注（I/R）损伤是急性肾衰竭的主要原因。然而，炎症反应和细胞凋亡是 I/R 损伤中涉及的重要机制。最近的研究表明，核因子 kappa B（NF-кB）和肿瘤坏死因子 α（TNF-α）都参与了这些机制。七氟醚可降低大鼠心脏中 NF-кB 和 TNF-α 的表达，减轻其肾 I/R 损伤。然而，关于七氟醚对糖尿病大鼠肾脏的影响的研究较少。因此，本研究旨在评估七氟醚对糖尿病大鼠 NF-кB 和 TNF-α 表达的影响，以减轻肾 I/R 损伤。
方法
雄性 Sprague-Dawley 大鼠分为五组：A 组，非糖尿病大鼠行假手术；B 组，非糖尿病大鼠肾 I/R 损伤；C 组，糖尿病大鼠行假手术；D 组，糖尿病大鼠肾 I/R 损伤；E 组，糖尿病大鼠肾 I/R 损伤后七氟醚预处理。E 组大鼠暴露于 2.5%七氟醚 30 min。24 h 后，评估大鼠肌酐（Cr）、血尿素氮（BUN）、肾细胞凋亡以及肾脏 NF-кB 和 TNF-α 的表达。
结果
与行假手术的糖尿病大鼠相比，糖尿病大鼠肾 I/R 损伤组的肾细胞凋亡、NF-кB 和 TNF-α 的表达明显升高（P<0.01）。七氟醚可显著降低这些变化（P<0.01）。
结论
七氟醚通过降低糖尿病大鼠肾 I/R 损伤中 NF-кB 和 TNF-α 的表达，对肾损伤发挥保护作用。
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