全反式维甲酸对肾缺血再灌注损伤糖尿病大鼠的影响。
Effects of ATRA on diabetic rats with renal ischemia-reperfusion injury.
作者信息
Cheng Zeng, Qian Sun, Qingtao Meng, Zhongyuan Xia, Yeda Xiao
机构信息
MM, Department of Anesthesiology, People's Hospital of Wuhan University, Wuhan, Hubei, China. Conception and design of the study, analysis and interpretation of data, manuscript preparation.
PhD, Department of Anesthesiology, People's Hospital of Wuhan University, Wuhan, Hubei, China. Acquisition and analysis of data.
出版信息
Acta Cir Bras. 2020 Mar 27;35(1):e202000106. doi: 10.1590/s0102-865020200010000006. eCollection 2020.
PURPOSE
To explore the role of all-trans retinoic acid (ATRA) in renal ischemia/reperfusion injury of diabetic rats.
METHODS
Sixty adult male rats were randomly divided into 6 groups, including sham group (S group), ischemia-reperfusion group (I/R group), ischemia-reperfusion+ATRA group (A group), diabetic group (D group), diabetic ischemia-reperfusion group (DI/R group), diabetic ischemia-reperfusion +ATRA group (DA group). The levels of creatinine (Cr), cystatin C (Cys-C) and β2-microglobulin (β2-MG) were measured. Morphology of renal tissue was observed under light microscope.
RESULTS
DJ-1, Nrf2, HO-1 and caspase-3 were detected by western blot. DJ-1, Nrf2, HO-1 and caspase-3 in I/R group, D group and DI/R group was higher than that in S group. Compared with I/R group, Nrf2 and HO-1 in A group was decreased, but caspase-3 was increased. However, Nrf2 in DA group was higher than that in DI/R group, HO-1 and caspase-3 in DA group were lower than that in DI/R group. Compared with group S, Cr, Cys-C and β2-MG in I/R group, A group, D group, and DI/R group were higher. Whereas the levels of Cr, Cys-C, β2-MG and renal injury score in DA group were lower than those in DI/R group.
CONCLUSION
ATRA has a protective effect on renal ischemia-reperfusion injury in diabetic rats, maybe relating to DJ/Nrf2 pathway.
目的
探讨全反式维甲酸（ATRA）在糖尿病大鼠肾脏缺血/再灌注损伤中的作用。
方法
将60只成年雄性大鼠随机分为6组，包括假手术组（S组）、缺血-再灌注组（I/R组）、缺血-再灌注+ATRA组（A组）、糖尿病组（D组）、糖尿病缺血-再灌注组（DI/R组）、糖尿病缺血-再灌注+ATRA组（DA组）。检测肌酐（Cr）、胱抑素C（Cys-C）和β2-微球蛋白（β2-MG）水平。在光学显微镜下观察肾组织形态。
结果
通过蛋白质印迹法检测DJ-1、Nrf2、HO-1和caspase-3。I/R组、D组和DI/R组中的DJ-1、Nrf2、HO-1和caspase-3高于S组。与I/R组相比，A组中的Nrf2和HO-1降低，但caspase-3升高。然而，DA组中的Nrf2高于DI/R组，DA组中的HO-1和caspase-3低于DI/R组。与S组相比，I/R组、A组、D组和DI/R组中的Cr、Cys-C和β2-MG更高。而DA组中的Cr、Cys-C、β2-MG水平和肾损伤评分低于DI/R组。
结论
ATRA对糖尿病大鼠肾脏缺血-再灌注损伤具有保护作用，可能与DJ/Nrf2通路有关。
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