Ozbilgin Sule, Ozkardesler Sevda, Akan Mert, Boztas Nilay, Ozbilgin Mucahit, Ergur Bekir Ugur, Derici Serhan, Guneli Mehmet Ensari, Meseri Reci
Department of Anesthesiology and Reanimation, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
Department of General Surgery, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
Biomed Res Int. 2016;2016:8580475. doi: 10.1155/2016/8580475. Epub 2016 Jan 26.
The aim of this study was to evaluate the effects of local ischemic preconditioning using biochemical markers and histopathologically in the diabetic rat renal IR injury model.
DM was induced using streptozotocin. Rats were divided into four groups: Group I, nondiabetic sham group (n = 7), Group II, diabetic sham group (n = 6), Group III, diabetic IR group (diabetic IR group, n = 6), and Group IV, diabetic IR + local ischemic preconditioning group (diabetic IR + LIPC group, n = 6). Ischemic renal injury was induced by clamping the bilateral renal artery for 45 min. 4 h following ischemia, clearance protocols were applied to assess biochemical markers and histopathologically in rat kidneys.
The histomorphologic total cell injury scores of the nondiabetic sham group were significantly lower than diabetic sham, diabetic IR, and diabetic IR + LIPC groups. Diabetic IR group scores were not significantly different than the diabetic sham group. But diabetic IR + LIPC group scores were significantly higher than the diabetic sham and diabetic IR groups.
Local ischemic preconditioning does not reduce the risk of renal injury induced by ischemia/reperfusion in diabetic rat model.
本研究旨在利用生化标志物和组织病理学方法，评估局部缺血预处理对糖尿病大鼠肾脏缺血再灌注损伤模型的影响。
使用链脲佐菌素诱导糖尿病。将大鼠分为四组：第一组，非糖尿病假手术组（n = 7）；第二组，糖尿病假手术组（n = 6）；第三组，糖尿病缺血再灌注组（糖尿病缺血再灌注组，n = 6）；第四组，糖尿病缺血再灌注+局部缺血预处理组（糖尿病缺血再灌注+局部缺血预处理组，n = 6）。通过夹闭双侧肾动脉45分钟诱导缺血性肾损伤。缺血4小时后，采用清除方案评估大鼠肾脏的生化标志物和组织病理学情况。
非糖尿病假手术组的组织形态学总细胞损伤评分显著低于糖尿病假手术组、糖尿病缺血再灌注组和糖尿病缺血再灌注+局部缺血预处理组。糖尿病缺血再灌注组的评分与糖尿病假手术组无显著差异。但糖尿病缺血再灌注+局部缺血预处理组的评分显著高于糖尿病假手术组和糖尿病缺血再灌注组。
在糖尿病大鼠模型中，局部缺血预处理并不能降低缺血/再灌注诱导的肾损伤风险。
