Ghafouri Bijar, Carlsson Anders, Holmberg Sara, Thelin Anders, Tagesson Christer
Division of Community Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Center, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland, Linköping, SE-581 85, Sweden.
Division of Neuro and Inflammation Science, Department of Clinical and Experimental Medicine, Linköping University, Occupational and Environmental Medicine Center, Heart and Medicine Center, Region Östergötland, Linköping, Sweden.
BMC Musculoskelet Disord. 2016 May 10;17:206. doi: 10.1186/s12891-016-1059-y.
Farmers have an increased risk for musculoskeletal disorders (MSD) such as osteoarthritis of the hip, low back pain, and neck and upper limb complaints. The underlying mechanisms are not fully understood. Work-related exposures and inflammatory responses might be involved. Our objective was to identify plasma proteins that differentiated farmers with MSD from rural referents.
Plasma samples from 13 farmers with MSD and rural referents were included in the investigation. Gel based proteomics was used for protein analysis and proteins that differed significantly between the groups were identified by mass spectrometry.
In total, 15 proteins differed significantly between the groups. The levels of leucine-rich alpha-2-glycoprotein, haptoglobin, complement factor B, serotransferrin, one isoform of kininogen, one isoform of alpha-1-antitrypsin, and two isoforms of hemopexin were higher in farmers with MSD than in referents. On the other hand, the levels of alpha-2-HS-glycoprotein, alpha-1B-glycoprotein, vitamin D- binding protein, apolipoprotein A1, antithrombin, one isoform of kininogen, and one isoform of alpha-1-antitrypsin were lower in farmers than in referents. Many of the identified proteins are known to be involved in inflammation.
Farmers with MSD had altered plasma levels of protein biomarkers compared to the referents, indicating that farmers with MSD may be subject to a more systemic inflammation. It is possible that the identified differences of proteins may give clues to the biochemical changes occurring during the development and progression of MSD in farmers, and that one or several of these protein biomarkers might eventually be used to identify and prevent work-related MSD.
农民患肌肉骨骼疾病（MSD）的风险增加，如髋骨关节炎、腰痛以及颈部和上肢疾病。其潜在机制尚未完全明确。可能涉及与工作相关的暴露和炎症反应。我们的目的是鉴定能够区分患有MSD的农民与农村对照者的血浆蛋白。
研究纳入了13名患有MSD的农民和农村对照者的血浆样本。基于凝胶的蛋白质组学用于蛋白质分析，通过质谱鉴定两组之间有显著差异的蛋白质。
两组之间共有15种蛋白质存在显著差异。患有MSD的农民中，富含亮氨酸的α-2-糖蛋白、触珠蛋白、补体因子B、血清转铁蛋白、一种激肽原异构体、一种α-1-抗胰蛋白酶异构体以及两种血红素结合蛋白异构体的水平高于对照者。另一方面，农民体内α-2-HS-糖蛋白、α-1B-糖蛋白、维生素D结合蛋白、载脂蛋白A1、抗凝血酶、一种激肽原异构体和一种α-1-抗胰蛋白酶异构体的水平低于对照者。许多已鉴定出的蛋白质已知与炎症有关。
与对照者相比，患有MSD的农民血浆中蛋白质生物标志物水平发生了改变，这表明患有MSD的农民可能存在更广泛的炎症。所鉴定出的蛋白质差异有可能为农民MSD发生和发展过程中出现的生化变化提供线索，并且这些蛋白质生物标志物中的一种或几种最终可能用于识别和预防与工作相关的MSD。
