Department of Neurosurgery, Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital, Jeonju, South Korea.
Connect Tissue Res. 2013;54(1):76-81. doi: 10.3109/03008207.2012.746320. Epub 2012 Dec 3.
Carpal tunnel syndrome (CTS) is the most common peripheral nerve entrapment, causing pain, impairment, and disability. To identify proteins of CTS comprehensively, a comparative serum analysis of CTS patients and normal control subjects was performed. The two-dimensional electrophoresis patterns of serum obtained from six CTS patients and six normal control subjects were compared. We found 10 proteins that were significantly altered in the serum of CTS patients, among which four were upregulated and six were downregulated. The upregulated spots were identified as Chain A, heat shock 70-kDa protein, 42-kDa ATPase N-terminal domain; glutathione-insulin transhydrogenase (216AA); cAMP-dependent protein kinase inhibitor alpha; and mutant β-globin. The downregulated spots were identified as vitamin D-binding protein (VDBP), fibrinogen gamma chain, apolipoprotein A-IV (ApoA-IV), clusterin, heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1), and one unidentified protein. The information obtained from this proteomic analysis will be very useful in understanding the pathophysiology of CTS and in finding suitable proteins that can serve as new diagnostic biomarkers of CTS.
腕管综合征（CTS）是最常见的周围神经嵌压症，可引起疼痛、功能障碍和残疾。为了全面鉴定 CTS 的蛋白，我们对 CTS 患者和正常对照者的血清进行了对比分析。比较了 6 例 CTS 患者和 6 例正常对照者的血清双向电泳图谱，发现 CTS 患者血清中有 10 种蛋白明显改变，其中 4 种上调，6 种下调。上调的斑点被鉴定为链 A、热休克 70kDa 蛋白、42kDaATPaseN-末端结构域、谷胱甘肽-胰岛素转氢酶（216AA）、cAMP 依赖性蛋白激酶抑制剂α和突变β-球蛋白。下调的斑点被鉴定为维生素 D 结合蛋白（VDBP）、纤维蛋白原γ链、载脂蛋白 A-IV（ApoA-IV）、簇蛋白、异质核核糖核蛋白 H1（hnRNP H1）和一个未知蛋白。这项蛋白质组学分析所获得的信息将非常有助于理解 CTS 的病理生理学，并找到合适的蛋白作为 CTS 的新的诊断生物标志物。
