加拿大飞蓬甲醇提取物对脂多糖（LPS）刺激的RAW264.7小鼠巨噬细胞的抗炎作用。
Anti-inflammatory effect of methanolic extract of Conyzacanadensis in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells.
作者信息
Song Jia-Le, Yi Ruo-Kun, Gao Yang
机构信息
Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi, People's Republic of China / Department of Food Science and Nutrition, Pusan National University, Busan, South Korea.
Department of Food Science and Nutrition, Pusan National University, Busan, South Korea.
出版信息
Pak J Pharm Sci. 2016 May;29(3):935-40.
The aim of this study was to investigate the potential anti-inflammatory effect of Conyzacanadeusis methanol extract (CME) using a cell model of RAW264.7 murine macrophage cell stimulated with lipopolysaccharide (LPS)(1μg/ml). Co-treatment with different concentrations (10, 50 and 100μg/ml) of CME was concentration-dependently reduced the LPS-induced generation of prostaglandin E2 (PGE2), nitric oxide (NO) tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, CME also reduced the mRNA expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), TNF-α, IL-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggested that CME showed an anti-inflammatory activity through reduced the mRNA expression of COX-2, iNOS, TNF-α IL-1β and IL-6 and also decreased the productions of PGE2, NO, TNF-α IL-1β and IL-6in LPS-stimulated RAW264.7 cells.
本研究的目的是使用脂多糖（LPS，1μg/ml）刺激的RAW264.7小鼠巨噬细胞模型，研究南美蟛蜞菊甲醇提取物（CME）的潜在抗炎作用。用不同浓度（10、50和100μg/ml）的CME共同处理，可浓度依赖性地降低LPS诱导的前列腺素E2（PGE2）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和IL-6的生成。此外，CME还降低了LPS刺激的RAW264.7细胞中环氧合酶-2（COX-2）、诱导型一氧化氮合酶（iNOS）、TNF-α、IL-1β和IL-6的mRNA表达。这些结果表明，CME通过降低COX-2、iNOS、TNF-α、IL-1β和IL-6的mRNA表达以及减少LPS刺激的RAW264.7细胞中PGE2、NO、TNF-α、IL-1β和IL-6的产生而表现出抗炎活性。
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