State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
Department of Mechanical Engineering, Faculty of Engineering and Department of Biochemistry & Genetics, Faculty of Medicine and Manitoba Institute of Child Health, University of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
ACS Appl Mater Interfaces. 2016 Jun 1;8(21):13242-50. doi: 10.1021/acsami.6b01432. Epub 2016 May 18.
Treating bone defects in the presence of infection is a formidable clinical challenge. The use of a biomaterial with the dual function of bone regeneration and infection control is a novel therapeutic approach to this problem. In this study, we fabricated an innovative, dual-function biocomposite hydrogel containing nanosilver and nanosilica (nAg/nSiO2) particles and evaluated its characteristics using FT-IR, SEM, swelling ratio, and stiffness assays. The in vitro antibacterial analysis showed that this nAg/nSiO2 hydrogel inhibited both Gram-positive and Gram-negative bacteria. In addition, this nontoxic material could promote osteogenic differentiation of rat bone marrow stromal cells (BMSCs). We then created infected bone defects in rat calvaria in order to evaluate the function of the hydrogel in vivo. The hydrogel demonstrated effective antibacterial ability while promoting bone regeneration in these defects. Our results indicate that this nAg/nSiO2 hydrogel has the potential to both control infection and to promote bone healing in contaminated defects.
在感染存在的情况下治疗骨缺损是一个艰巨的临床挑战。使用具有骨再生和感染控制双重功能的生物材料是解决这一问题的新治疗方法。在这项研究中，我们制备了一种含有纳米银和纳米硅（nAg/nSiO2）颗粒的创新型双重功能的生物复合水凝胶，并通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、溶胀比和硬度测试评估了其特性。体外抗菌分析表明，这种 nAg/nSiO2 水凝胶抑制了革兰氏阳性菌和革兰氏阴性菌。此外，这种无毒材料可以促进大鼠骨髓基质细胞（BMSCs）的成骨分化。然后，我们在大鼠颅骨中制造了感染性骨缺损，以评估水凝胶在体内的功能。水凝胶在促进这些缺损中的骨再生的同时表现出有效的抗菌能力。我们的结果表明，这种 nAg/nSiO2 水凝胶具有控制感染和促进污染缺损中骨愈合的潜力。
