Ding Xinxin, Shi Junyu, Wei Jianxu, Li Yuan, Wu Xiangbing, Zhang Yi, Jiang Xue, Zhang Xiaomeng, Lai Hongchang
Department of Oral Implantology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China.
Sci Adv. 2021 Dec 10;7(50):eabj7857. doi: 10.1126/sciadv.abj7857.
Composite hydrogels incorporating natural polymers and bioactive glass (BG) are promising materials for bone regeneration. However, their applications are compromised by the poor interfacial compatibility between organic and inorganic phases. In this study, we developed an electrostatically reinforced hydrogel (CAG) with improved interfacial compatibility by introducing amino-functionalized 45S5 BG to the alginate/gellan gum (AG) matrix. BAG composed of AG and unmodified BG (10 to 100 μm in size) was prepared as a control. Compared with BAG, CAG had a more uniform porous structure with a pore size of 200 μm and optimal compressive strength of 66 kPa. Furthermore, CAG promoted the M2 phenotype transition of macrophages and up-regulated the osteogenic gene expression of stem cells. The new bone formation in vivo was also accelerated due to the enhanced biomineralization of CAG. Overall, this work suggests CAG with improved interfacial compatibility is an ideal material for bone regeneration application.
包含天然聚合物和生物活性玻璃（BG）的复合水凝胶是用于骨再生的有前景的材料。然而，它们的应用因有机相和无机相之间较差的界面相容性而受到影响。在本研究中，我们通过将氨基官能化的45S5 BG引入藻酸盐/结冷胶（AG）基质中，开发了一种具有改善的界面相容性的静电增强水凝胶（CAG）。制备了由AG和未改性BG（尺寸为10至100μm）组成的BAG作为对照。与BAG相比，CAG具有更均匀的多孔结构，孔径为200μm，最佳抗压强度为66 kPa。此外，CAG促进巨噬细胞的M2表型转变，并上调干细胞的成骨基因表达。由于CAG增强的生物矿化作用，体内新骨形成也加速了。总体而言，这项工作表明具有改善的界面相容性的CAG是骨再生应用的理想材料。
