滑石粉胸膜固定术治疗癌性胸膜炎在动物模型中的实验验证
Experimental validation of talc pleurodesis for carcinomatous pleuritis in an animal model.
作者信息
Iwasaki Yasunori, Takamori Shinzo, Mitsuoka Masahiro, Kashihara Masaki, Nishi Tatsuya, Murakami Daigo, Matsumoto Ryoichi, Mifune Hiroharu, Tajiri Yuji, Akagi Yoshito
机构信息
Department of Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan.
Institute of Animal Experimentation, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan.
出版信息
Gen Thorac Cardiovasc Surg. 2016 Jul;64(7):409-13. doi: 10.1007/s11748-016-0653-0. Epub 2016 May 12.
OBJECTIVE
This study was performed to evaluate the change of pleural cavity by talc pleurodesis for carcinomatous pleuritis in an animal model.
METHODS
Models of malignant pleuritis were produced by an intra-venous injection of lung adenocarcinoma cells to male BALB/c nude mice at 6 weeks of age. Two weeks after the injection, either talc or saline was injected into the left thoracic cavity and the mice were further observed for 4 weeks. Six weeks after the injection, they were killed and the occurrence of lung cancer, amount of pleural effusion, and histological change of the pleural cavity were examined and compared between the two groups with or without talc administration.
RESULTS
Talc administration caused a significant reduction in pleural effusion with no increase of pleural adhesion. Talc administration resulted in marked pleural thickening compared to saline treatment. The vascular architecture and its area did not differ between the two groups.
CONCLUSION
Talc pleurodesis to reduce pleural effusion is valid for carcinomatous pleuritis, potentially through an acceleration of pleural thickening.
目的
本研究旨在评估滑石粉胸膜固定术对动物模型中癌性胸膜炎胸腔变化的影响。
方法
通过向6周龄雄性BALB/c裸鼠静脉注射肺腺癌细胞来制备恶性胸膜炎模型。注射两周后，将滑石粉或生理盐水注入左胸腔，并对小鼠进一步观察4周。注射6周后，处死小鼠，检查两组（给予或未给予滑石粉）的肺癌发生情况、胸腔积液量以及胸腔的组织学变化，并进行比较。
结果
滑石粉给药导致胸腔积液显著减少，且胸膜粘连未增加。与生理盐水治疗相比，滑石粉给药导致胸膜明显增厚。两组之间的血管结构及其面积没有差异。
结论
滑石粉胸膜固定术减少胸腔积液对癌性胸膜炎有效，可能是通过加速胸膜增厚实现的。
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