人三叉神经节解离细胞中的甲型疱疹病毒DNA复制
Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia.
作者信息
Cohrs Randall J, Badani Hussain, Bos Nathan, Scianna Charles, Hoskins Ian, Baird Nicholas L, Gilden Don
机构信息
Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.
出版信息
J Neurovirol. 2016 Oct;22(5):688-694. doi: 10.1007/s13365-016-0450-7. Epub 2016 May 12.
Abstract
Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological replicates of cells from dissociated randomly distributed human trigeminal ganglia (TG) of four subjects revealed an increase in both parameters and in both viruses during 5 days of culture, with no further change by 10 days. Dissociated TG provides a platform to analyze initiation of latent virus DNA replication within 5 days of culture.
摘要
对来自四名受试者随机分布的人三叉神经节（TG）的解离细胞的多个生物学重复样本中的单纯疱疹病毒1型（HSV-1）和水痘带状疱疹病毒（VZV）DNA的频率和PCR可定量丰度进行分析，结果显示在培养5天期间，两种病毒的这两个参数均增加，到10天时没有进一步变化。解离的TG提供了一个平台，用于分析培养5天内潜伏病毒DNA复制的起始情况。
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