Sasaki Yuichi, Sasaki Masanori, Kataoka-Sasaki Yuko, Nakazaki Masahito, Nagahama Hiroshi, Suzuki Junpei, Tateyama Daiki, Oka Shinichi, Namioka Takahiro, Namioka Ai, Onodera Rie, Mikami Takeshi, Wanibuchi Masahiko, Kakizawa Masafumi, Ishiai Sumio, Kocsis Jeffery D, Honmou Osamu
Y. Sasaki, PT, PhD, Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan, and Department of Rehabilitation, Sapporo Medical University School of Medicine.
M. Sasaki, MD, PhD, Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, 060-8556, Japan; Department of Neurology, Yale University School of Medicine, New Haven, Connecticut; and Center for Neuroscience and Regeneration Research, VA Connecticut Healthcare System, West Haven, Connecticut.
Phys Ther. 2016 Nov;96(11):1791-1798. doi: 10.2522/ptj.20150504. Epub 2016 May 12.
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia.
The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats.
This was an experimental study.
A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test.
Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group.
A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.
The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model.
静脉输注源自成人骨髓的间充质干细胞（MSCs）可改善大鼠中风模型的行为功能。通过体育锻炼进行的康复治疗对脑缺血也具有治疗效果。
本研究旨在探讨每日康复训练与静脉输注MSCs的协同作用对大鼠中风后是否具有治疗效果。
这是一项实验研究。
采用微丝血管腔内闭塞法诱导永久性大脑中动脉闭塞（MCAO）。研究了四个实验组：第1组（仅给予赋形剂，n = 10），第2组（赋形剂 + 运动，n = 10），第3组（仅给予MSCs，n = 10），第4组（MSCs + 运动，n = 10）。在MCAO后6小时静脉输注大鼠MSCs，并且大鼠每天接受20分钟的跑步机跑步康复训练。在第1、14和35天使用磁共振成像评估损伤大小。使用肢体放置试验评估功能结局。
与未输注MSCs的组相比，联合治疗和MSCs输注均减少了损伤体积，诱导了突触形成，并引起了功能改善，但联合治疗组的效果更佳。
本研究的一个局限性是结果仅限于动物模型，不能推广到人类。
数据表明，每日康复训练与静脉输注MSCs的联合治疗改善了大鼠MCAO模型的功能结局。
