Lukacova Jana, Jambor Tomas, Knazicka Zuzana, Tvrda Eva, Kolesarova Adriana, Lukac Norbert
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture , Nitra , Slovak Republic.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(7):669-76. doi: 10.1080/10934529.2015.1011963.
The present study was to evaluate the effect of bisphenol A (BPA) at the doses 1, 10, 100 and 200 µg mL(-1) on the bovine spermatozoa motility, viability and production of superoxide radical. The CASA system was used to determine the spermatozoa motility. The initial motility showed the significant differences (P < 0.001) between the groups higher than 100 µg BPA mL(-1) and the control group. Evaluation of the spermatozoa motility after 6 h of cultivation at the doses > 10 µg BPA mL(-1) was found to decrease motility significantly. After 24 h it was observed that the doses < 10 µg BPA mL(-1) statistically increased motility, while the doses > 100 µg BPA mL(-1) significantly decreased motility in comparison to control. The viability of spermatozoa as detected by the MTT assay decreased in all experimental groups, but significant differences were noted only at the highest doses of BPA after 24 h of in vitro cultivation. The intracellular superoxide production was observed by the NBT test after 24 h of BPA exposure. The results indicated that in all experimental groups the amount of superoxide increased as compared to the control group; significant changes were observed at the doses > 100 µg BPA mL(-1). In conclusion, the results from our experiments suggest the negative effects of BPA at the highest doses used on motility and viability of bovine spermatozoa and production of superoxide radical.
本研究旨在评估双酚A（BPA）在1、10、100和200 µg mL(-1)剂量下对牛精子活力、生存力和超氧自由基产生的影响。使用计算机辅助精子分析（CASA）系统测定精子活力。初始活力显示，高于100 µg BPA mL(-1)的组与对照组之间存在显著差异（P < 0.001）。发现在>10 µg BPA mL(-1)剂量下培养6小时后，精子活力显著下降。24小时后观察到，<10 µg BPA mL(-1)剂量在统计学上提高了活力，而>100 µg BPA mL(-1)剂量与对照组相比显著降低了活力。通过MTT法检测，所有实验组的精子生存力均下降，但仅在体外培养24小时后，最高剂量的BPA组出现显著差异。在BPA暴露24小时后，通过NBT试验观察细胞内超氧产生情况。结果表明，与对照组相比，所有实验组的超氧量均增加；在>100 µg BPA mL(-1)剂量下观察到显著变化。总之，我们的实验结果表明，所用最高剂量的BPA对牛精子的活力、生存力和超氧自由基产生具有负面影响。
