Sameni Hamid Reza, Ramhormozi Parisa, Bandegi Ahmad Reza, Taherian Abbas Ali, Mirmohammadkhani Majid, Safari Manouchehr
Research Center of Nervous System Stem Cell, Department of Anatomical Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Research Center of Nervous System Stem Cell, Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
J Diabetes Investig. 2016 Jul;7(4):506-13. doi: 10.1111/jdi.12459. Epub 2016 May 28.
AIMS/INTRODUCTION: Oxidative stress has a key role in the pathogenesis of diabetes. Propolis and its constituents have a wide range of medicinal properties against oxidative stress. In the present study, we evaluated the anti-oxidant effects of ethanolic extracts of propolis on kidneys in diabetes mellitus rats.
A total of 40 male Wistar rats were randomly divided into the following five groups: control, diabetes mellitus, diabetes mellitus with vehicle treatment, diabetes mellitus with propolis treatment (100 mg/kg) and diabetes mellitus with propolis treatment (200 mg/kg). Diabetes mellitus in rats was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Diabetic groups were treated with vehicle or ethanolic extracts of Iranian propolis for 6 weeks. Serum concentration of malondialdehyde, superoxide dismutase and glutathione peroxidase were measured.
The results showed that Iranian propolis significantly inhibited bodyweight loss in diabetes mellitus rats. The propolis extracts significantly reduced serum glucose levels and kidney weight in diabetes mellitus rats (P < 0.001). Furthermore, propolis extracts significantly reduced the malondialdehyde content, and increased the activity of superoxide dismutase and glutathione peroxidase (P < 0.001) along with the total anti-oxidant activity in the kidney tissue of diabetes mellitus rats. In the kidneys of the diabetes mellitus and vehicle group, the glomerular basement membrane thickness and glomerular area were significantly increased. Treatment of diabetes mellitus rats with the propolis extract significantly reduced the glomerular basement membrane thickness and glomerular area.
The present study results showed that the Iranian propolis extract could enhance the anti-oxidant levels and histopathological changes in the kidneys of rats. The final results showed that most of the favorable effects of propolis are mediated by a reduction of blood glucose levels in diabetic animals.
目的/引言：氧化应激在糖尿病发病机制中起关键作用。蜂胶及其成分具有广泛的抗氧化应激药用特性。在本研究中，我们评估了蜂胶乙醇提取物对糖尿病大鼠肾脏的抗氧化作用。
总共40只雄性Wistar大鼠被随机分为以下五组：对照组、糖尿病组、糖尿病溶媒治疗组、糖尿病蜂胶治疗组（100毫克/千克）和糖尿病蜂胶治疗组（200毫克/千克）。通过腹腔注射链脲佐菌素（60毫克/千克）诱导大鼠患糖尿病。糖尿病组用溶媒或伊朗蜂胶乙醇提取物治疗6周。测量血清丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶浓度。
结果表明，伊朗蜂胶显著抑制糖尿病大鼠体重减轻。蜂胶提取物显著降低糖尿病大鼠的血糖水平和肾脏重量（P<0.001）。此外，蜂胶提取物显著降低丙二醛含量，增加超氧化物歧化酶和谷胱甘肽过氧化物酶活性（P<0.001），同时提高糖尿病大鼠肾脏组织的总抗氧化活性。在糖尿病组和溶媒组大鼠的肾脏中，肾小球基底膜厚度和肾小球面积显著增加。用蜂胶提取物治疗糖尿病大鼠可显著降低肾小球基底膜厚度和肾小球面积。
本研究结果表明，伊朗蜂胶提取物可提高大鼠肾脏的抗氧化水平并改善组织病理学变化。最终结果表明，蜂胶的大多数有益作用是通过降低糖尿病动物的血糖水平来介导的。
