激光扫描显微镜中的最佳透镜设计与应用。
Optimal lens design and use in laser-scanning microscopy.
作者信息
Negrean Adrian, Mansvelder Huibert D
机构信息
Integrative Neurophysiology, Centre for Neurogenomics and Cognitive Research, VU University, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands ; Neuroscience Campus Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.
Integrative Neurophysiology, Centre for Neurogenomics and Cognitive Research, VU University, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands ; Neuroscience Campus Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands ;
出版信息
Biomed Opt Express. 2014 Apr 18;5(5):1588-609. doi: 10.1364/BOE.5.001588. eCollection 2014 May 1.
In laser-scanning microscopy often an off-the-shelf achromatic doublet is used as a scan lens which can reduce the available diffraction-limited field-of-view (FOV) by a factor of 3 and introduce chromatic aberrations that are scan angle dependent. Here we present several simple lens designs of superior quality that fully make use of high-NA low-magnification objectives, offering diffraction-limited imaging over a large FOV and wavelength range. We constructed a two-photon laser-scanning microscope with optimized custom lenses which had a near diffraction limit point-spread-function (PSF) with less than 3.6% variation over a 400 µm FOV and less than 0.5 µm lateral color between 750 and 1050 nm.
在激光扫描显微镜中，通常使用现成的消色差双合透镜作为扫描透镜，这会使可用的衍射极限视场（FOV）减小3倍，并引入与扫描角度相关的色差。在此，我们展示了几种高质量的简单透镜设计，它们充分利用了高数值孔径、低放大倍数的物镜，在大视场和波长范围内提供衍射极限成像。我们构建了一台配备优化定制透镜的双光子激光扫描显微镜，其具有接近衍射极限的点扩散函数（PSF），在400 µm视场内变化小于3.6%，在750至1050 nm之间横向色差小于0.5 µm。
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