Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, California, USA.
Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
Nat Methods. 2016 Jul;13(7):569-72. doi: 10.1038/nmeth.3866. Epub 2016 May 16.
Genetically encoded calcium sensors have enabled monitoring of neural activity in vivo using optical imaging techniques. Linking neural activity to complex behavior remains challenging, however, as most imaging systems require tethering the animal, which can impact the animal's behavioral repertoire. Here, we report a method for monitoring the brain activity of untethered, freely walking Drosophila melanogaster during sensorially and socially evoked behaviors to facilitate the study of neural mechanisms that underlie naturalistic behaviors.
基因编码的钙传感器使人们能够使用光学成像技术在体内监测神经活动。然而，将神经活动与复杂行为联系起来仍然具有挑战性，因为大多数成像系统需要将动物系绳，这会影响动物的行为表现。在这里，我们报告了一种在感官和社交诱发行为期间监测未系绳、自由行走的黑腹果蝇大脑活动的方法，以促进对自然行为背后的神经机制的研究。
