Alves Patrícia, Santos Marta, Mendes Sabrina, P Miguel Sónia, D de Sá Kevin, S D Cabral Cátia, J Correia Ilídio, Ferreira Paula
CIEPQPF, Department of Chemical Engineering, Universidade de Coimbra, P-3030 790 Coimbra, Portugal.
CICS-UBI, Health Sciences Research Center, Universidade da Beira Interior, P-6200 506 Covilhã, Portugal.
Polymers (Basel). 2019 Apr 10;11(4):653. doi: 10.3390/polym11040653.
Recently, the biomedical scientists who are working in the skin regeneration area have proposed asymmetric membranes as ideal wound dressings, since they are able to reproduce both layers of skin and improve the healing process as well as make it less painful. Herein, an electrospinning technique was used to produce new asymmetric membranes. The protective layer was composed of a blending solution between polycaprolactone and polylactic acid, whereas the underlying layer was comprised of methacrylated gelatin and chitosan. The chemical/physical properties, the in vitro hemo- and biocompatibility of the nanofibrous membranes were evaluated. The results obtained reveal that the produced membranes exhibited a wettability able to provide a moist environment at wound site. Moreover, the membranes' hemocompatibility and fibroblast cell adhesion, spreading and proliferation at the surface of the membranes were also noticed in the in vitro assays. Such results highlight the suitability of these asymmetric membranes for wound dressing applications.
最近，从事皮肤再生领域研究的生物医学科学家提出，不对称膜是理想的伤口敷料，因为它们能够再生皮肤的两层结构，改善愈合过程，并减轻疼痛。在此，采用静电纺丝技术制备了新型不对称膜。保护层由聚己内酯和聚乳酸的混合溶液组成，而底层由甲基丙烯酸化明胶和壳聚糖组成。对纳米纤维膜的化学/物理性质、体外血液相容性和生物相容性进行了评估。所得结果表明，所制备的膜具有润湿性，能够在伤口部位提供湿润环境。此外，在体外试验中还观察到了膜的血液相容性以及成纤维细胞在膜表面的粘附、铺展和增殖。这些结果突出了这些不对称膜在伤口敷料应用中的适用性。
