Kotturi Hari, Abuabed Alaeddin, Zafar Haris, Sawyer Elaine, Pallipparambil Bipin, Jamadagni Harsha, Khandaker Morshed
Department of Biology, University of Central Oklahoma, Edmond, OK 73034, USA.
Department of Engineering & Physics, University of Central Oklahoma, Edmond, OK 73034, USA.
J Funct Biomater. 2017 Sep 5;8(3):39. doi: 10.3390/jfb8030039.
Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold.
厚度超过1毫米且不存在营养管道网络的聚乙二醇二丙烯酸酯（PEGDA）组织支架，由于细胞无法在支架内粘附和迁移，在组织工程中的应用有限。已在PEGDA支架的两侧涂覆聚己内酯（PCL）电纺纳米纤维（ENF）膜以克服这些限制，从而创建一种功能性PEGDA-PCL支架。本研究检测了PEGDA和PEGDA-PCL支架的物理、力学和生物学特性，以确定PCL涂层对PEGDA的影响。PEGDA-PCL样品的物理表征证明了将PCL与PEGDA支架相结合以扩大其在组织工程中应用的有效性。该研究还发现，由于添加了PCL层，PEGDA的弹性有显著提高。本研究表明，PEGDA-PCL支架会随时间吸收营养物质，并能为细胞存活提供理想环境。此外，细胞活力测试表明细胞在PEGDA-PCL支架中粘附、增殖和迁移。因此，PCL ENF涂层对PEGDA支架有积极影响。
