Cha In-Tae, Park Inhye, Lee Hae-Won, Lee Hyunjin, Park Jung-Min, Roh Seong Woon, Choi Hak-Jong, Nam Young-Do, Lee Yoo Kyung, Seo Myung-Ji
Division of Bioengineering, Incheon National University, 22012 Incheon, Republic of Korea.
Department of Life Sciences, Graduate School of Incheon National University, 22012 Incheon, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Aug;66(8):3125-3131. doi: 10.1099/ijsem.0.001156. Epub 2016 May 17.
A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain MME-001T, was isolated from the tidal flat of Muui-do in the Republic of Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001T belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35T (98.0 % similarity of the 16S rRNA gene). Growth of strain MME-001T occurred in the presence of 1.0-7.0 % (w/v) NaCl at 15-40 °C and pH 7.0-9.0, with optimal growth in the presence of 2.0-3.0 % (w/v) NaCl at 25-30 °C and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The major cellular fatty acids were C18 : 1ω7c and C16 : 0. The genomic DNA G+C content was 62 mol%. DNA-DNA hybridization values between strain MME-001T and P. sabulilitoris KCTC 42111T, 'Pseudoruegeria limi' KCTC 32460, Pseudoruegeria lutimaris KCTC 22690T, Pseudoruegeria aquimaris KCTC 12737T and Pseudoruegeriahaliotis KACC 17214T was 36±5, 57±7, 34±4, 18±5 and 21±3 %, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain MME-001T represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain is MME-001T (=KCCM 43133T=JCM 30751T).
从韩国牟伊岛的潮滩中分离出一株革兰氏阴性、无运动性、需氧的杆状细菌，命名为MME-001T。基于16S rRNA基因序列构建的系统发育树表明，菌株MME-001T属于红杆菌科的假鲁杰氏菌属，并且它与沙质假鲁杰氏菌GJMS-35T的16S rRNA基因序列相似度最高（16S rRNA基因相似度为98.0%）。菌株MME-001T在1.0-7.0%（w/v）NaCl、15-40°C和pH 7.0-9.0条件下生长，在2.0-3.0%（w/v）NaCl、25-30°C和pH 7.0条件下生长最佳。泛醌-10是主要的呼吸醌。主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的脂质。主要细胞脂肪酸为C18:1ω7c和C16:0。基因组DNA G+C含量为62 mol%。菌株MME-001T与沙质假鲁杰氏菌KCTC 42111T、“海滨假鲁杰氏菌”KCTC 32460、泥质假鲁杰氏菌KCTC 22690T、海生假鲁杰氏菌KCTC 12737T和鲍氏假鲁杰氏菌KACC 17214T之间的DNA-DNA杂交值分别为36±5%、57±7%、34±4%、18±5%和21±3%。基于表型和系统发育分类学特征，该菌株MME-001T代表假鲁杰氏菌属的一个新物种，为此提出新名称海滨假鲁杰氏菌（Pseudoruegeria aestuarii sp. nov.）。模式菌株为MME-001T（=KCCM 43133T=JCM 30751T）。
