Cha In-Tae, Cho Eui-Sang, Choi Hak-Jong, Roh Seong Woon, Seo Myung-Ji
1Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
2Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon 22012, Republic of Korea.
Int J Syst Evol Microbiol. 2019 May;69(5):1465-1471. doi: 10.1099/ijsem.0.003345. Epub 2019 Mar 14.
A Gram-stain-negative, obligate aerobic, non-motile and rod-shaped bacterium, designated MME-070, was isolated from a tidal flat near Muui-do, Incheon, Republic of Korea. This bacterium belonged to the Roseobacterclade within the family Rhodobacteraceae. The isolate grew at 20-40 °C, pH 7.0-9.0 with 1-5 % (w/v) NaCl. Optimal growth required 2-3 % (w/v) NaCl at 25-30 °C and pH 8.0. The 16S rRNA gene sequence of strain MME-070 was most closely related to Roseovarius aestuarii SMK-122 (96.9 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MME-070 clustered with the genus Roseovarius. The sole respiratory quinone was Q-10. Polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, unidentified aminolipid and some unidentified lipids. Major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), 11-methyl C18 : 1ω7c and C16 : 0. The genomic DNA G+C content of the type strain was 63.6 mol%. Based on polyphasic taxonomic analysis, strain MME-070 is proposed to represent a novel species of the genus Roseovarius, for which the name Roseovarius faecimaris sp. nov. is proposed. The type strain is MME-070 (=KCCM 43142=JCM 30757).
从韩国仁川舞衣岛附近的潮滩中分离出一株革兰氏染色阴性、专性需氧、无运动性的杆状细菌，命名为MME-070。该细菌属于红杆菌科内的玫瑰杆菌属。该分离株在20-40°C、pH 7.0-9.0和1-5%（w/v）NaCl条件下生长。最适生长条件为25-30°C、pH 8.0和2-3%（w/v）NaCl。菌株MME-070的16S rRNA基因序列与河口玫瑰杆菌SMK-122最为相似（96.9%）。基于16S rRNA基因序列的系统发育分析表明，菌株MME-070与玫瑰杆菌属聚为一类。唯一的呼吸醌为Q-10。极性脂质为磷脂酰甘油、磷脂酰胆碱、二磷脂酰甘油、未鉴定的氨基脂质和一些未鉴定的脂质。主要细胞脂肪酸为总和特征8（C18:1ω7c和/或C18:1ω6c）、11-甲基C18:1ω7c和C16:0。模式菌株的基因组DNA G+C含量为63.6 mol%。基于多相分类分析，建议菌株MME-070代表玫瑰杆菌属的一个新物种，命名为粪便玫瑰杆菌（Roseovarius faecimaris sp. nov.）。模式菌株为MME-070（=KCCM 43142=JCM 30757）。
