使用超薄贝塞尔光片的多色 4D 荧光显微镜。
Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets.
机构信息
Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
出版信息
Sci Rep. 2016 May 18;6:26159. doi: 10.1038/srep26159.
We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.
我们展示了一种简单而高效的方法，使用线光斑和环形滤波器产生任意颜色的超薄贝塞尔（“无衍射”）光束。利用这种稳健且经济高效的技术，我们获得了生物样品的双色、3D 图像，其横向/轴向分辨率分别为 250nm/400nm，以及以 1Hz 时间分辨率对 20μm 大小的活样本进行高速、4D 体积成像。
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