Hosseinkhani Faride, Jabalameli Fereshteh, Banar Maryam, Abdellahi Nafiseh, Taherikalani Morovat, Leeuwen Willem B van, Emaneini Mohammad
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
Rev Soc Bras Med Trop. 2016 Apr;49(2):172-6. doi: 10.1590/0037-8682-0329-2015.
The aim of this study was to determine whether an herbal extract containing monoterpene exhibited activity against multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa isolated from clinical infection samples.
The essential oil of Trachyspermum ammi (L.) Sprague ex Turrill (Apiaceae) fruit was extracted by hydrodistillation. Fruit residues were treated with hydrochloric acid and re-hydrodistilled to obtain volatile compounds. Compounds in the distilled oil were identified using gas-chromatography (GC) and GC-mass spectrometry (MS). The antibiotic susceptibility of all bacterial isolates was analyzed using both the disc diffusion method and determination of the minimum inhibitory concentration (MIC). The sensitivity of antibiotic-resistant isolates to essential oil was also determined by using the disc diffusion method and MIC determination.
Of 26 clinical isolates, 92% were multidrug-resistant (MDR). Aromatic monoterpenes (thymol, paracymene, and gamma-terpinene) were the major (90%) components of the oil. Growth of S. aureus strains was successfully inhibited by the oil, with an inhibitory zone diameter (IZD) between 30-60mm and MIC <0.02μL/mL. The oil had no antimicrobial activity against clinical isolates of P. aeruginosa; rather, it prevented pigment production in these isolates.
This study revealed that the essential oil of Trachyspermum ammi, which contains monoterpene, has good antibacterial potency. Monoterpenes could thus be incorporated into antimicrobial ointment formulas in order to treat highly drug-resistant S. aureus infections. Our findings also underscore the utility of research on natural products in order to combat bacterial multidrug resistance.
本研究的目的是确定一种含有单萜的草药提取物对从临床感染样本中分离出的耐多药金黄色葡萄球菌和铜绿假单胞菌是否具有活性。
采用水蒸馏法提取藏茴香（伞形科）果实的精油。果实残渣用盐酸处理后再进行水蒸馏以获得挥发性化合物。使用气相色谱（GC）和GC-质谱（MS）对蒸馏油中的化合物进行鉴定。采用纸片扩散法和最低抑菌浓度（MIC）测定法分析所有细菌分离株的抗生素敏感性。还通过纸片扩散法和MIC测定法确定耐抗生素分离株对精油的敏感性。
在26株临床分离株中，92%为耐多药（MDR）菌株。芳香单萜（百里香酚、对异丙基甲苯和γ-萜品烯）是该油的主要（90%）成分。该油成功抑制了金黄色葡萄球菌菌株的生长，抑菌圈直径（IZD）在30 - 60mm之间，MIC<0.02μL/mL。该油对铜绿假单胞菌临床分离株没有抗菌活性；相反，它阻止了这些分离株产生色素。
本研究表明，含有单萜的藏茴香精油具有良好的抗菌效力。因此，单萜可被纳入抗菌软膏配方中，以治疗高度耐药的金黄色葡萄球菌感染。我们的研究结果还强调了天然产物研究在对抗细菌多重耐药性方面的实用性。
