Grădinaru A C, Trifan A, Şpac A, Brebu M, Miron A, Aprotosoaie A C
Department of Animal and Vegetal Biology, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Iasi, Romania.
Department of Pharmacognosy, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Iasi, Romania.
Lett Appl Microbiol. 2018 Nov;67(5):449-457. doi: 10.1111/lam.13069.
The aim of this study was to investigate the effects of ajowan essential oil (AjEO)/thymol and antibiotics combinations against three standard strains and six resistant clinical isolates of major respiratory bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pneumoniae). The broth microdilution method was conducted to determine the minimum inhibitory concentrations (MIC) of essential oil/thymol and antibiotics. The checkerboard method was used to investigate the interactions between the essential oil/thymol and antibiotics by means of the fractional inhibitory concentration index (FICI). The chemical composition of essential oil was also analysed by GC-MS and GC-FID. Thymol (50·75%), γ-terpinene (25·94%) and p-cymene (18·31%) were identified as major constituents of the oil. The most sensitive organisms to ajowan volatile oil were Strep. pneumoniae bacteria (MIC = 0·125-0·5 mg ml ). Synergistic effects were observed with AjEO/thymol and amoxicillin combinations on methicillin-resistant Staph. aureus clinical isolates (FICI = 0·37-0·50) and with essential oil and ciprofloxacin combinations against P. aeruginosa ATCC 27853, Staph. aureus ATCC 25923 and penicillin (P)-resistant Strep. pneumoniae bacteria (FICI = 0·37-0·50). Combination of thymol and ciprofloxacin produces synergistic effects only against P. aeruginosa ATCC 27853 and P-resistant Strep. pneumoniae clinical isolate (FICI = 0·46-0·49).
本研究旨在调查阿育吠陀精油（AjEO）/百里香酚与抗生素联合使用对三种标准菌株以及六种主要呼吸道细菌（铜绿假单胞菌、金黄色葡萄球菌和肺炎链球菌）耐药临床分离株的影响。采用肉汤微量稀释法测定精油/百里香酚和抗生素的最低抑菌浓度（MIC）。通过棋盘法，借助分数抑菌浓度指数（FICI）研究精油/百里香酚与抗生素之间的相互作用。还采用气相色谱 - 质谱联用（GC - MS）和气相色谱 - 氢火焰离子化检测器（GC - FID）分析了精油的化学成分。鉴定出百里香酚（50·75%）、γ - 萜品烯（25·94%）和对伞花烃（18·31%）为该精油的主要成分。对阿育吠陀挥发油最敏感的微生物是肺炎链球菌（MIC = 0·125 - 0·5 mg/ml）。观察到AjEO/百里香酚与阿莫西林联合使用对耐甲氧西林金黄色葡萄球菌临床分离株具有协同作用（FICI = 0·37 - 0·50），精油与环丙沙星联合使用对铜绿假单胞菌ATCC 27853、金黄色葡萄球菌ATCC 25923和耐青霉素（P）肺炎链球菌具有协同作用（FICI = 0·37 - 0·50）。百里香酚与环丙沙星联合使用仅对铜绿假单胞菌ATCC 27853和耐P肺炎链球菌临床分离株产生协同作用（FICI = 0·46 - 0·49）。
