Altenburger W, Süter C P, Altenburger J
Central Research Units, F. Hoffmann-La Roche & Co., Basel, Switzerland.
Arch Virol. 1989;105(1-2):15-27. doi: 10.1007/BF01311113.
The genome of a strongly attenuated vaccinia virus strain, MVA, was investigated by Southern blot and sequence analyses. Three major deletions, relative to the WR strain, were localized in MVA DNA. The deletions occurred near both ends of the viral genome and one of them affected a 55 K as well as the 32 K human host range gene. Although more than two thirds of the host range gene were eliminated from the MVA, the virus could still multiply in certain human cells.
通过Southern印迹法和序列分析研究了一种强减毒痘苗病毒株MVA的基因组。相对于WR株，MVA DNA中有三个主要缺失区域。这些缺失发生在病毒基因组的两端附近，其中一个缺失影响了55K以及32K人宿主范围基因。尽管MVA中超过三分之二的宿主范围基因被删除，但该病毒仍能在某些人类细胞中增殖。
