Sziegoleit A, Knäpler H, Peters B
Institute of Medical Microbiology, University of Giessen, FRG.
Clin Biochem. 1989 Apr;22(2):79-83. doi: 10.1016/s0009-9120(89)80002-5.
A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for the RIA. In addition, the ELISA could be valid as a screening test for cystic fibrosis. Because ELISA reagents are relatively stable, the assay is always ready to use and can be performed without special equipment.
开发了一种用于检测人胰腺弹性蛋白酶1的夹心型酶联免疫吸附测定（ELISA），并评估了其临床可行性。使用高度纯化的试剂，该检测对于检测血清弹性蛋白酶1水平升高似乎与记录完善的放射免疫测定（RIA）一样灵敏和特异，因此，其适用指征与RIA相同。此外，ELISA可作为囊性纤维化的筛查检测方法。由于ELISA试剂相对稳定，该检测随时可以进行，且无需特殊设备。
