van Rijn J L, Schmidt N A, Rutten W P
Stichting Medische Laboratoria, Breda, The Netherlands.
Clin Chem. 1989 May;35(5):840-3.
As recommended by the World Health Organization, standardization of prothrombin time assays involves conversion of prothrombin times into International Normalized Ratios (INR). We investigated the effect of two different methods (Nycomed's Thrombotest, and Instrumentation Laboratory's PT-fibrinogen) and three coagulation instruments (Schnitger & Gross, KC-10, and ACL) on calculations of INR. The INR plots showed considerable scatter of individual values around the regression lines when the two different methods were compared. Systematic differences in the outcome of INR calculation were related to the use of the different coagulation instruments. Prothrombin times obtained with the different instruments were linearly correlated. We used the bias of these lines to correct results for both the patients' samples and the reference samples. This correction yielded INR values from the different instruments that agreed well.
按照世界卫生组织的建议，凝血酶原时间测定的标准化涉及将凝血酶原时间转换为国际标准化比值（INR）。我们研究了两种不同方法（Nycomed公司的血栓试验和仪器实验室的PT-纤维蛋白原法）以及三种凝血仪器（施尼特格&格罗斯公司、KC-10和ACL）对INR计算的影响。当比较两种不同方法时，INR图显示各个值在回归线周围有相当大的离散度。INR计算结果的系统差异与不同凝血仪器的使用有关。用不同仪器获得的凝血酶原时间呈线性相关。我们利用这些直线的偏差对患者样本和参考样本的结果进行校正。这种校正得出不同仪器的INR值非常一致。
