猪蛔虫中超氧化物歧化酶的物理化学特性

Physico-chemical characteristics of superoxide dismutase in Ascaris suum.

Sanchez-Moreno M, Garcia-Ruiz M A, Sanchez-Navas A, Monteoliva M

Instituto Lopez-Neyra de Parasitologia Sección de Bioquimica, Granada, Spain.

Comp Biochem Physiol B. 1989;92(4):737-40. doi: 10.1016/0305-0491(89)90259-9.

Abstract

Three SOD isoenzymes obtained from purified extracts of Ascaris suum were characterized. 2. The physico-chemical characteristics studied were: optimum pH, methods of preservation of enzymatic activity, molecular weight, and the u.v. and visible light absorption spectra. 3. The optimum pH for the Cu, Zn SOD I and II was 10.2 and 10.1 for the Mn SOD. 4. The extracts retained their levels of activity longer at -70 degrees C, and after lyophilization. The Mn SOD was more labile than the Cu, Zn SOD I and II. 5. The molecular weights obtained by filtration through Sephadex G-75 were: 73,000 for Mn SOD; 42,600 for Cu, Zn SOD I; and 39,800 for the Cu, Zn SOD II. 6. Both the u.v. and visible light spectra were similar to other dismutases from other sources.

摘要

对从猪蛔虫纯化提取物中获得的三种超氧化物歧化酶（SOD）同工酶进行了表征。2. 所研究的物理化学特性包括：最适pH值、酶活性的保存方法、分子量以及紫外和可见光吸收光谱。3. 铜锌SOD I和II的最适pH值为10.2，锰SOD的最适pH值为10.1。4. 提取物在-70℃以及冻干后能更长时间保持其活性水平。锰SOD比铜锌SOD I和II更不稳定。5. 通过Sephadex G-75过滤获得的分子量分别为：锰SOD为73,000；铜锌SOD I为42,600；铜锌SOD II为39,800。6. 紫外光谱和可见光谱均与其他来源的其他歧化酶相似。