Ali Roozegar Mohamad, Azizi Jalilian Farid, Reza Havasian Mohamad, Panahi Jafar, Pakzad Iraj
Department of Periodentistry, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam/Iran.
Department Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan/ Iran.
Bioinformation. 2016 Jan 31;12(1):19-21. doi: 10.6026/97320630012019. eCollection 2016.
The antimicrobial effect of the mastic tree (Pistacia atlantica) under in vitro conditions has been reported. Therefore, it is of interest to evaluate the effect of the plant leaf extract (aqueous) on bacterial load in mouth and saliva. The leaf of the Pistacia atlantica plant was collected and cleaned, dried at 40⁰c and then powdered. The extraction was carried out using the maceration method in vacuum with the rotary evaporator device. Bacterial inhibition (Streptococcus species) by the leaf extract was studied using the disc diffusion and embedding sink diffusion methods. The values of MIC and MBC were determined. The collected data was further analyzed using t-test and repeated measure statistical tests. The disc diffusion technique showed a significant inhibitory effect for Pistacia atlantica leaf extract on S. mutans (ATCC 35668) and S. mitis (ATCC 49456) with inhibition zones of 19 and 25 millimeters, respectively. This is for the highest leaf extract concentration used in this study (p<0.01). The values of MIC and MBC for S.mutans was 60, 90 μg/ml and for S. mitis was 75, 110 μg/ml (p<0.01 significance). The leaf extract has no significant effect on S. salivarius (ATCC 13419). Thus, the antimicrobial properties of the aqueous leaf extract from Pistacia atlantica is demonstrated in this study.
乳香黄连木（Pistacia atlantica）在体外条件下的抗菌作用已有报道。因此，评估该植物叶提取物（水提取物）对口腔和唾液中细菌载量的影响具有重要意义。收集乳香黄连木叶并清洗，在40℃下干燥，然后研磨成粉。使用旋转蒸发仪通过浸渍法在真空中进行提取。采用纸片扩散法和包埋水槽扩散法研究叶提取物对细菌（链球菌属）的抑制作用。测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值。使用t检验和重复测量统计检验对收集的数据进行进一步分析。纸片扩散技术显示，乳香黄连木叶提取物对变形链球菌（ATCC 35668）和缓症链球菌（ATCC 49456）具有显著抑制作用，抑菌圈分别为19毫米和25毫米。这是针对本研究中使用的最高叶提取物浓度而言（p<0.01）。变形链球菌的MIC和MBC值分别为60、90μg/ml，缓症链球菌的MIC和MBC值分别为75、110μg/ml（p<0.01，具有显著性）。叶提取物对唾液链球菌（ATCC 13419）没有显著影响。因此，本研究证明了乳香黄连木叶水提取物的抗菌特性。
