Judaki Arezo, Panahi Jafar, Havasian Mohamad Reza, Tajbakhsh Parnian, Roozegar Mohamad Ali
Department of Gastroenterology, Ilam University of Medical Sciences, Ilam/ Iran.
Student research of committee, Ilam University of Medical Sciences, Ilam/Iran.
Bioinformation. 2014 Nov 27;10(11):689-92. doi: 10.6026/97320630010689. eCollection 2014.
The use of therapeutic herbs has become of great importance these days due to the increase in drug resistance. From a long time ago the Venus' navel plant has been used to treat infections. In this study the antibacterial effect of the aqueous extract from the Quercus coccifera (jaft) herb, under laboratory conditions. This study was carried out experimentally. After collecting the Venus navel herb, it was dried in a warm dry environment away from direct sunlight in the shade. The alcoholic extract was prepared using a standard method. Clinical samples of staphylococcus aureus and pseudomonas aeruginosa were acquired from Ilam's health care institutes. The inhibitory effect of the extracts was analysed in the Mueller Hinton using the disk diffusion method for both bacteria. Then MIC and MBC of the extracts was determined using the Macro broth dilution method. At its highest concentration the aqueous extract had an inhibition zone of 27.2 and 23.7 mm on staphylococcus aureus and pseudomonas aeruginosa consecutively. The MIC and MBC for staphylococcus aureus were 10 and 12.5 µg/ml and for pseudomonas aeruginosa they were 10 and 17.5 µg/m consecutively. The results of this study show the strong antimicrobial effect of jaft's aqueous extract on staphylococcus aureus and pseudomonas aeruginosa and if more studies are based on this topic it could be a substitute for common antibiotics.
由于耐药性的增加,如今治疗性草药的使用变得极为重要。从很久以前起,金星草就被用于治疗感染。在本研究中,在实验室条件下研究了胭脂虫栎(jaft)草药水提取物的抗菌作用。本研究通过实验进行。采集金星草后,将其置于温暖干燥、避光的阴凉环境中晾干。采用标准方法制备乙醇提取物。金黄色葡萄球菌和铜绿假单胞菌的临床样本取自伊拉姆的医疗机构。使用纸片扩散法在 Mueller Hinton 培养基中分析提取物对两种细菌的抑制作用。然后使用常量肉汤稀释法测定提取物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。水提取物在最高浓度时,对金黄色葡萄球菌和铜绿假单胞菌的抑菌圈直径分别为 27.2 和 23.7 毫米。金黄色葡萄球菌的 MIC 和 MBC 分别为 10 和 12.5 µg/ml,铜绿假单胞菌的 MIC 和 MBC 分别为 10 和 17.5 µg/ml。本研究结果表明 jaft 水提取物对金黄色葡萄球菌和铜绿假单胞菌具有较强的抗菌作用,如果基于该主题进行更多研究,它可能会成为常用抗生素的替代品。