State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005069.
Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320/zg-336, zg-917/zg-910 and zg-913/zg-915) isolated from animal tissues and human faeces were found to belong to the genus based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320/zg-336 had the highest 16S rRNA gene similarity to DSM 44353 (97.51 %), zg-917/zg-910 to DSM 44184 (98.68 %), and zg-913/zg-915 to subsp. CIP 103500 (98.79 %). The three novel type strains had a relatively high DNA G+C content (61.2-64.4 mol%), low DNA relatedness and ANI values with their respective neighbours: 23.5/72.7 %, 25.0/72.3%and 22.6/73.1 % (zg-320 vs. CIP 106629, DSM 45100 and DSM 45110); 24.4/82.3% and 23.7/81.3 % (zg-917 vs. DSM 44184 and JCB); 26.8/83.7% and 27.7/84.4 % (zg-913 vs. ATCC 700355 and subsp. CCUG 32105). The three novel species had C, C, C 9 and C ante/C 6,9 as the major cellular fatty acids; MK-8(H) in strain zg-917 and MK-9(H) in strains zg-320 and zg-913 were found to be the major respiratory quinones. For the three novel species, the detected major polar lipids included diphosphatidylglycerol, phosphatidyl inositol mannoside, phosphatidylglycerol and phosphatidylinositol, the cell-wall peptidoglycan was based on -DAP, and the whole-cell sugars mainly included ribose, arabinose and galactose. The three novel species grew optimally at 35-37 °C, 0.5 % (w/v) NaCl and pH 7.0-8.0; notably, they were tolerant of 10.5 % (w/v) NaCl. Based on the results of these comprehensive analyses, three novel species in the genus are proposed, aptly named sp. nov. (zg-320 = GDMCC 1.1719 = JCM 34106), sp. nov. (zg-917 = GDMCC 1.1707 = JCM 34094) and sp. nov. (zg-913 = GDMCC 1.1706 = JCM 34398).
从动物组织和人类粪便中分离到的六株兼性厌氧、革兰氏阳性、杆状、非溶血的细菌（zg-320/zg-336、zg-917/zg-910 和 zg-913/zg-915），根据 16S rRNA 基因和 262 个核心基因集的系统发育分析，属于 属。根据 16S rRNA 相似性最大程度，zg-320/zg-336 与 DSM 44353（97.51%）具有最高的 16S rRNA 基因相似性，zg-917/zg-910 与 DSM 44184（98.68%），zg-913/zg-915 与 subsp. CIP 103500（98.79%）。三个新的模式株具有相对较高的 DNA G+C 含量（61.2-64.4 mol%）、较低的 DNA 相关性和与各自邻居的 ANI 值：23.5/72.7%、25.0/72.3%和 22.6/73.1%（zg-320 与 CIP 106629、DSM 45100 和 DSM 45110；24.4/82.3%和 23.7/81.3%（zg-917 与 DSM 44184 和 JCB）；26.8/83.7%和 27.7/84.4%（zg-913 与 ATCC 700355 和 subsp. CCUG 32105）。三个新种具有 C16:0、C16:1ω7c 和 C18:1ω7c、ante/C6 9 作为主要的细胞脂肪酸；在菌株 zg-917 中发现 MK-8（H）和在菌株 zg-320 和 zg-913 中发现 MK-9（H）是主要的呼吸醌。对于三个新种，检测到的主要极性脂包括双磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰甘油和磷脂酰肌醇，细胞壁肽聚糖基于 -DAP，全细胞糖主要包括核糖、阿拉伯糖和半乳糖。三个新种在 35-37°C、0.5%（w/v）NaCl 和 pH 7.0-8.0 条件下生长最佳；值得注意的是，它们能耐受 10.5%（w/v）NaCl。基于这些综合分析的结果，提出了属中的三个新种，分别命名为 sp. nov.（zg-320=GDMCC 1.1719=JCM 34106）、 sp. nov.（zg-917=GDMCC 1.1707=JCM 34094）和 sp. nov.（zg-913=GDMCC 1.1706=JCM 34398）。
