Yang Xiaochen, Steinberg Florian, Zhuang Lei, Bessey Ralph, Trueb Beat
Department of Clinical Research, University of Bern, CH-3008 Bern, Switzerland.
Int J Mol Med. 2016 Jul;38(1):30-8. doi: 10.3892/ijmm.2016.2601. Epub 2016 May 23.
Fibroblast growth factor receptor (FGFR)-like protein 1 (FGFRL1) is the most recently discovered member of the FGFR family. Owing to the fact that it interacts with FGF ligands, but lacks the intracellular tyrosine kinase domain, several researchers have speculated that it may function as a decoy receptor and exert a negative effect on cell proliferation. In this study, we performed overexpression experiments with TetOn‑inducible cell clones and downregulation experiments with siRNA oligonucleotides, and found that FGFRL1 had absolutely no effect on cell growth and proliferation. Likewise, we did not observe any influence of FGFRL1 on ERK1/2 activation and on the phosphorylation of 250 other signaling proteins analyzed by the Kinexus antibody microarray. On the other hand, with bacterial petri dishes, we observed a clear effect of FGFRL1 on cell adhesion during the initial hours after cell seeding. Our results suggest that FGFRL1 is a cell adhesion protein similar to the nectins rather than a signaling receptor similar to FGFR1-FGFR4.
成纤维细胞生长因子受体（FGFR）样蛋白1（FGFRL1）是FGFR家族中最新发现的成员。由于它能与FGF配体相互作用，但缺乏细胞内酪氨酸激酶结构域，一些研究人员推测它可能作为一种诱饵受体发挥作用，并对细胞增殖产生负面影响。在本研究中，我们对TetOn诱导性细胞克隆进行了过表达实验，并用小干扰RNA（siRNA）寡核苷酸进行了下调实验，发现FGFRL1对细胞生长和增殖完全没有影响。同样，我们也未观察到FGFRL1对ERK1/2激活以及通过Kinexus抗体微阵列分析的其他250种信号蛋白磷酸化有任何影响。另一方面，在细菌培养皿中，我们观察到FGFRL1在细胞接种后的最初几个小时对细胞黏附具有明显作用。我们的结果表明，FGFRL1是一种类似于nectins的细胞黏附蛋白，而非类似于FGFR1 - FGFR4的信号受体。
