Amin Kamal Adel, Hashem Khalid Shaban, Alshehri Fawziah Saleh, Awad Said T, Hassan Mohammed S
Department of Chemistry, College of Science, University of Dammam, PO Box 383, Dammam, 31113, Dammam, Saudi Arabia.
Biochemistry Department, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef City, Egypt.
Biol Trace Elem Res. 2017 Jan;175(1):136-145. doi: 10.1007/s12011-016-0748-6. Epub 2016 May 25.
Overdoses of acetaminophen (APAP), a famous and widely used drug, may have hepatotoxic effects. Nanoscience is a novel scientific discipline that provides specific tools for medical science problems including using nano trace elements in hepatic diseases. Our study aimed to assess the hepatoprotective role of selenium nanoparticles (Nano-Se) against APAP-induced hepatic injury. Twenty-four male rats were classified into three equal groups: a control group that received 0.9 % NaCl, an APAP-treated group (oral administration), and a group treated with Nano-Se (10-20 nm, intraperitoneal (i.p.) injection) and APAP (oral administration). APAP overdose induced significant elevations in liver function biomarkers, hepatic lipid peroxidation, hepatic catalase, and superoxide dismutase (SOD), decreased the reduced glutathione (GSH) content and glutathione reductase (GR) activity, and stimulated significant DNA damage in hepatocytes, compared to control rats. Nano-Se administration improved the hepatic antioxidant protection mechanism and decreased cellular sensitivity to DNA fragmentation. Nano-Se exhibits a protective effect against APAP-induced hepatotoxicity through improved liver function and oxidative stress mediated by catalase, SOD, and GSH and decreases hepatic DNA fragmentation, a hepatic biomarker of cell death. Nano-Se could be a novel hepatoprotective strategy to inhibit oxidative stress.
对乙酰氨基酚（APAP）是一种广为人知且广泛使用的药物，过量服用可能会产生肝毒性作用。纳米科学是一门新兴的科学学科，它为医学问题提供了特定工具，包括在肝脏疾病中使用纳米微量元素。我们的研究旨在评估硒纳米颗粒（Nano-Se）对APAP诱导的肝损伤的肝脏保护作用。将24只雄性大鼠分为三组，每组数量相等：一组为接受0.9%氯化钠的对照组，一组为APAP治疗组（口服给药），另一组为用Nano-Se（10 - 20纳米，腹腔注射）和APAP（口服给药）治疗的组。与对照大鼠相比，APAP过量导致肝功能生物标志物、肝脂质过氧化、肝过氧化氢酶和超氧化物歧化酶（SOD）显著升高，还原型谷胱甘肽（GSH）含量和谷胱甘肽还原酶（GR）活性降低，并刺激肝细胞出现显著的DNA损伤。给予Nano-Se改善了肝脏抗氧化保护机制，并降低了细胞对DNA片段化的敏感性。Nano-Se通过改善肝功能以及由过氧化氢酶、SOD和GSH介导的氧化应激，对APAP诱导的肝毒性发挥保护作用，并减少肝DNA片段化，这是细胞死亡的一种肝脏生物标志物。Nano-Se可能是一种抑制氧化应激的新型肝脏保护策略。
