Mansoori Behzad, Mohammadi Ali, Shirjang Solmaz, Baghbani Elham, Baradaran Behzad
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail :
Asian Pac J Cancer Prev. 2016;17(4):1887-90. doi: 10.7314/apjcp.2016.17.4.1887.
Breast cancer is the most common cause of cancer-related death among women in the whole world. MiR- 34a and let-7a are well known tumor suppressors that participate in the regulation of apoptosis, invasion and other cellular functions. In this study, expression of miR-34a, let-7a and apoptosis pathway genes such as Bcl-2, Caspase-3 and P53 were evaluated using quantitative real-time PCR in 45 paired samples of normal margin and tumor tissue collected from breast cancer patient at advanced stage (3-4). MiR-34a, let-7a, caspase-3 and P53 expression are reduced and Bcl-2 expression is increased within tumoral tissues in comparison with normal margin tissues. P53 expression directly or indirectly was correlated with miR-34a, let-7a, Bcl-2 and caspase-3 expression. In This study we found that MiR-34a and let-7a expression are reduced in the tumoral tissues. Down- regulation of these two molecules correlated with expression of genes associated with apoptosis. These results suggest that due to the correlation of miR-34a and let-7a with apoptotic and anti-apoptotic pathways these molecules could participate as regulators in advanced clinical stages of breast cancer and should be considered as markers for diagnosis, prognostic assessment and targeted therapy.
乳腺癌是全球女性癌症相关死亡的最常见原因。MiR-34a和let-7a是众所周知的肿瘤抑制因子，参与细胞凋亡、侵袭及其他细胞功能的调节。在本研究中，采用定量实时PCR对45对取自晚期（3 - 4期）乳腺癌患者的正常边缘组织和肿瘤组织样本进行检测，评估MiR-34a、let-7a及凋亡通路相关基因如Bcl-2、Caspase-3和P53的表达。与正常边缘组织相比，肿瘤组织中MiR-34a、let-7a、Caspase-3和P53的表达降低，而Bcl-2的表达增加。P53的表达与MiR-34a、let-7a、Bcl-2和Caspase-3的表达直接或间接相关。在本研究中我们发现，肿瘤组织中MiR-34a和let-7a的表达降低。这两种分子的下调与凋亡相关基因的表达有关。这些结果表明，由于MiR-34a和let-7a与凋亡和抗凋亡通路相关，这些分子可能在晚期乳腺癌临床阶段作为调节因子发挥作用，应被视为诊断、预后评估和靶向治疗的标志物。
