Nehela Yasser, Hijaz Faraj, Elzaawely Abdelnaser A, El-Zahaby Hassan M, Killiny Nabil
Plant Pathology Department, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, United States; Department of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta, Egypt.
Plant Pathology Department, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, United States.
J Plant Physiol. 2016 Jul 20;199:12-17. doi: 10.1016/j.jplph.2016.04.005. Epub 2016 May 17.
Phytohormones mainly affect plant development and trigger varied responses to biotic and abiotic stresses. The sensitivity of methods used to profile phytohormones is a vital factor that affects the results. We used an improved GC-MS-based method in the selective ion-monitoring (SIM) mode to study the phytohormone profiling in citrus tissues. One extraction solvent mixture and two derivatization reagents were used, methyl chloroformate (MCF) and N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA). The method showed a low limit of detection and low limit of quantification with high extraction recovery percentage and reproducibility. Overall, we detected 13 phytohormones belonging to six different groups. Auxins, SAs, tJA, and ABA were detected after derivatization with MCF while cytokinins and GAs were detected after derivatization with MSTFA. Cytokinins, SAs, and gibberellins were found in all tissues while auxins and tJA were observed only in the leaves. ABA was found in leaves and roots, but not in root tips. The method we used is efficient, precise, and appropriate to study citrus phytohormonal profiles to understand their crosstalk and responses to environmental and biological stresses.
植物激素主要影响植物发育，并引发对生物和非生物胁迫的各种反应。用于分析植物激素的方法的灵敏度是影响结果的一个关键因素。我们采用了一种改进的基于气相色谱-质谱联用（GC-MS）的选择性离子监测（SIM）模式的方法，来研究柑橘组织中的植物激素谱。使用了一种提取溶剂混合物以及两种衍生化试剂，即氯甲酸甲酯（MCF）和N-甲基-N-（三甲基硅基）三氟乙酰胺（MSTFA）。该方法具有低检测限和低定量限，提取回收率高且重现性好。总体而言，我们检测到了属于六个不同类别的13种植物激素。用MCF衍生化后检测到生长素、水杨酸（SAs）、反式茉莉酸（tJA）和脱落酸（ABA），而用MSTFA衍生化后检测到细胞分裂素和赤霉素（GAs）。在所有组织中都发现了细胞分裂素、SAs和赤霉素，而生长素和tJA仅在叶片中观察到。ABA在叶片和根中被发现，但在根尖中未发现。我们使用的方法高效、精确，适用于研究柑橘的植物激素谱，以了解它们之间的相互作用以及对环境和生物胁迫的反应。
