Tytor M, Franzén G, Olofsson J
Department of Otolaryngology, University Hospitals, Linköping, Sweden.
Head Neck. 1989 May-Jun;11(3):257-63. doi: 10.1002/hed.2880110312.
Single-cell DNA cytofluorometry was performed on paraffin-embedded tissue of 140 patients with squamous cell carcinomas of the oral cavity. Half of the tumors (71 of 140) were DNA nondiploid. Well-differentiated carcinomas were more often DNA diploid than moderately well-differentiated ones (P less than 0.001; chi-square). The aneuploid tumors responded better to preoperative radiotherapy than did the DNA diploid (P less than 0.001) and polyploid tumors (P less than 0.05; chi-square). Using the multivariate Cox's regression analysis multiploid type tumor, age of the patients and presence of lymph node metastases were the only significant factors influencing survival. DNA diploid tumors in stages I and II had a better prognosis than DNA nondiploid (P less than 0.01; Kaplan-Meier). The reverse was true for stages III and IV, where DNA nondiploid tumors had a better prognosis (P less than 0.05; Kaplan-Meier). Tumor stages (P less than 0.001; Kaplan-Meier) and especially lymph node metastases (P less than 0.0005; Kaplan-Meier) were major prognostic factors. Tumor DNA ploidy may be a complement to clinical and morphologic parameters as a prognostic predictor in squamous cell carcinoma of the oral cavity.
对140例口腔鳞状细胞癌患者的石蜡包埋组织进行了单细胞DNA细胞荧光测定。一半的肿瘤（140例中的71例）为DNA非二倍体。高分化癌比中分化癌更常为DNA二倍体（P<0.001；卡方检验）。非整倍体肿瘤对术前放疗的反应比DNA二倍体肿瘤（P<0.001）和多倍体肿瘤（P<0.05；卡方检验）更好。使用多变量Cox回归分析，多倍体类型肿瘤、患者年龄和淋巴结转移的存在是影响生存的唯一显著因素。I期和II期的DNA二倍体肿瘤比DNA非二倍体肿瘤预后更好（P<0.01；Kaplan-Meier法）。III期和IV期则相反，DNA非二倍体肿瘤预后更好（P<0.05；Kaplan-Meier法）。肿瘤分期（P<0.001；Kaplan-Meier法）尤其是淋巴结转移（P<0.0005；Kaplan-Meier法）是主要的预后因素。肿瘤DNA倍性可能作为口腔鳞状细胞癌预后预测指标，对临床和形态学参数起到补充作用。
