Moradi-Arzeloo Masoumeh, Farshid Amir Abbas, Tamaddonfard Esmaeal, Asri-Rezaei Siamak
Postgraduate student, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Vet Res Forum. 2016 Winter;7(1):47-54. Epub 2016 Mar 15.
In the present study, we investigated the effects of histidine and vitamin C (alone or in combination) treatments against isoproterenol (a β-adrenergic receptor agonist)-induced acute myocardial infarction in rats. We used propranolol (a β-adrenergic receptor blocker) to compare the results. Rats were given intraperitoneal injections of histidine (40 mg kg(-1)) and vitamin C (40 mg kg(-1)) alone and combined daily for 21 days. Propranolol (10 mg kg(-1)) was orally administered daily for 10 days (from day 11 to day 21). Myocardial infarction was induced by subcutaneous injections of 150 mg kg(-1) of isoproterenol at an interval of 24 hr on days 20 and 21. Blood and tissue samples were taken for histopathological and biochemical evaluations following electrocardiography recording on day 21. Isoproterenol elevated ST segment, increased heart weight, heart rate, serum activities of aspartate transaminase, lactate dehydrogenase, creatine kinase-MB and heart tissue content of malondialdehyde, and decreased R wave amplitude and superoxide dismutase and catalase activities of heart tissue. Necrosis, edema and inflammatory cells infiltration were observed in myocardial tissue sections. Our results indicated that histidine and vitamin C alone, and especially in combination prevent isoproterenol-induced cardiotoxicity and have similar protective effects with propranolol. Cardioprotective effects of histidine and vitamin C may be associated with their ability to reduce free radical-induced toxic effects.
在本研究中，我们调查了组氨酸和维生素C单独或联合处理对异丙肾上腺素（一种β-肾上腺素能受体激动剂）诱导的大鼠急性心肌梗死的影响。我们使用普萘洛尔（一种β-肾上腺素能受体阻滞剂）来比较结果。大鼠每天腹腔注射组氨酸（40 mg kg⁻¹）和维生素C（40 mg kg⁻¹）单独及联合用药，持续21天。普萘洛尔（10 mg kg⁻¹）每天口服给药10天（从第11天至第21天）。在第20天和第21天，每隔24小时皮下注射150 mg kg⁻¹异丙肾上腺素诱导心肌梗死。在第21天进行心电图记录后，采集血液和组织样本进行组织病理学和生化评估。异丙肾上腺素使ST段抬高，增加心脏重量、心率、天冬氨酸转氨酶、乳酸脱氢酶、肌酸激酶-MB的血清活性以及心脏组织丙二醛含量，并降低R波振幅以及心脏组织超氧化物歧化酶和过氧化氢酶活性。在心肌组织切片中观察到坏死、水肿和炎性细胞浸润。我们的结果表明，组氨酸和维生素C单独使用，尤其是联合使用可预防异丙肾上腺素诱导的心脏毒性，并且与普萘洛尔具有相似的保护作用。组氨酸和维生素C的心脏保护作用可能与其降低自由基诱导的毒性作用的能力有关。
