Huang L H, Silberman J, Rothschild H, Cohen J C
Department of Medicine, School of Medicine, Louisiana State University, New Orleans 70112-2822.
J Biol Chem. 1989 May 25;264(15):8811-4.
For the baboon endogenous virus to infect human cells a specific region on chromosome 6 is required for viral DNA replication and integration. In studying the kinetics of baboon endogenous virus DNA replication we show that linear DNA was synthesized as the predominant species after infection and that unintegrated DNA persisted after many cell passages. Examination of integrated DNA revealed the failure of the virus to integrate at early passages. With continuous replication, however, virus integration was observed, but at multiple sites in the host cell.
为了使狒狒内源性病毒感染人类细胞，病毒DNA复制和整合需要6号染色体上的一个特定区域。在研究狒狒内源性病毒DNA复制的动力学时，我们发现感染后线性DNA作为主要形式被合成，并且未整合的DNA在多次细胞传代后仍持续存在。对整合DNA的检查显示，病毒在早期传代时未能整合。然而，随着持续复制，观察到病毒整合，但发生在宿主细胞的多个位点。
