Lemons R S, Nash W G, O'Brien S J, Benveniste R E, Sherr C J
Cell. 1978 Aug;14(4):995-1005. doi: 10.1016/0092-8674(78)90353-7.
Human VA-2 cells infected with baboon type C virus were cloned and fused to Syrian hamster cells, and 33 primary hybrid colonies were obtained. These cells segregated human chromosomes and retained the complete hamster genome. Assays for type C viral p30 antigen and reverse transcriptase were performed in conjunction with analyses of 30 gene-enzyme systems representing 22 different human chromosomes. The results comfirmed that a gene, Bevi, previously assigned to human chromosome 6, dominantly controls baboon type C virus expression in hybrid cells. Representative hybrid colones were studied by nucleic acid hybridization techniques for the presence of integrated proviral DNA using complementary 3H-DNA transcripts of the baboon viral RNA genome. For each of 12 clones examined, there was a concordance between the presence of human chromosome 6, the presence of baboon type C proviral DNA sequences and virus expression. Clones which segregated chromosome 6 as judged by isozyme and karyological analyses lost detectable proviral DNA sequences and failed to produce virus. No syntenic association between the replication of baboon virus and the presence of 21 other human chromosomes was deteced. We conclude that Bevi is a preferred integration site for the baboon type C provirus in the human genome.
用狒狒C型病毒感染的人VA - 2细胞被克隆并与叙利亚仓鼠细胞融合，获得了33个初级杂交菌落。这些细胞分离出人类染色体并保留了完整的仓鼠基因组。进行了C型病毒p30抗原和逆转录酶检测，并结合对代表22条不同人类染色体的30个基因 - 酶系统的分析。结果证实，先前定位于人类6号染色体的基因Bevi在杂交细胞中显性控制狒狒C型病毒的表达。使用狒狒病毒RNA基因组的互补3H - DNA转录本，通过核酸杂交技术研究代表性杂交菌落中整合的前病毒DNA的存在情况。对于所检测的12个克隆中的每一个，人类6号染色体的存在、狒狒C型前病毒DNA序列的存在与病毒表达之间存在一致性。通过同工酶和染色体分析判断为分离出6号染色体的克隆失去了可检测到的前病毒DNA序列，并且未能产生病毒。未检测到狒狒病毒复制与其他21条人类染色体的存在之间的同线关联。我们得出结论，Bevi是狒狒C型前病毒在人类基因组中的一个优先整合位点。
