Vishnevskaya O N, Rybalchenko O V, Larionov I V, Orlova O G, Markov A G
Zh Mikrobiol Epidemiol Immunobiol. 2016 Mar-Apr(2):3-9.
Comparative study of tight junctions and ultrastructure alterations of enterocytes of mucous membranes of jejunum of rats under the effect of lipopolysaccharides and cholera toxin.
Lipopolysaccharides (Sigma-Aldrich, Germany) and cholera toxin (Sigma-Aldrich, Germany) were used. The study was carried out in Wistar line rats. Effect of lipopolysaccharides and cholera toxin on epitheliocytes was carried out by a method of withdrawal of segments of rat jejunum and their incubation with the specified substances. Comparative analysis of ultrathin sections of enterocytes of jejunum of rats and tight junctions between them was carried out in control and under the effect of lipopolysaccharides and cholera toxin.
Effect of lipopolysaccharides on ultrastructure of enterocytes of rat jejunum manifested in the change of cell form as a result of increase of intercellular space without destruction of tight junctions. Disappearance of desmosomes, increase of nuclei and more pronounced ER were noted in some epitheliocytes. Effect of cholerogen on epitheliocytes of mucous membrane of rat jejunum by a number of signs is similar to the effect of lipopolysaccharides, that manifested in an alteration of ultrastructure of cell, the form of those also transformed as a result of an increase of intercellular space, this process was not accompanied by destruction of tight junctions. Disappearance of folding of the lateral region of plasmatic membrane of cells and a reduction of a number of microvilli was observed under the effect of cholera toxin.
A similar character of effect of lipopolysaccharides and cholera toxins on ultrastructure of cells and region of tight junctions of enterocytes of rat jejunum was detected, both substances caused an increase of intercellular space without the destruction of tight junctions.
比较脂多糖和霍乱毒素作用下大鼠空肠黏膜肠上皮细胞紧密连接及超微结构的改变。
使用脂多糖（德国西格玛奥德里奇公司）和霍乱毒素（德国西格玛奥德里奇公司）。实验选用Wistar品系大鼠。通过取出大鼠空肠段并与特定物质孵育的方法，研究脂多糖和霍乱毒素对上皮细胞的作用。对大鼠空肠肠上皮细胞超薄切片及其之间的紧密连接进行对照以及在脂多糖和霍乱毒素作用下的比较分析。
脂多糖对大鼠空肠肠上皮细胞超微结构的影响表现为细胞形态改变，细胞间隙增大，但紧密连接未被破坏。部分上皮细胞中可见桥粒消失、细胞核增大以及内质网更明显。霍乱毒素对大鼠空肠黏膜上皮细胞的作用在许多方面与脂多糖相似，表现为细胞超微结构改变，细胞形态也因细胞间隙增大而转变，此过程未伴随紧密连接的破坏。在霍乱毒素作用下，观察到细胞膜外侧区域褶皱消失以及微绒毛数量减少。
检测到脂多糖和霍乱毒素对大鼠空肠肠上皮细胞超微结构及紧密连接区域的作用具有相似特征，两种物质均导致细胞间隙增大且紧密连接未被破坏。
