Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore; School of Biological Science, Nanyang Technological University, Singapore, Singapore.
Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore; School of Biological Science, Nanyang Technological University, Singapore, Singapore.
Adv Immunol. 2016;131:101-34. doi: 10.1016/bs.ai.2016.02.002. Epub 2016 Apr 8.
Advances of mass cytometry and high-dimensional single-cell data analysis have brought cellular immunological research into a new generation. By coupling these two powerful technology platforms, immunologists now have more tools to resolve the tremendous diversity of immune cell subsets, and their heterogeneous functionality. Since the first introduction of mass cytometry, many reports have been published using this novel technology to study a range of cell types. At the outset, studies of human hematopoietic stem cell and peripheral CD8(+) T cells using mass cytometry have shad the light of future experimental approach in interrogating immune cell phenotypic and functional diversity. Here, we briefly revisit the past and present understanding of T cell heterogeneity, and the technologies that facilitate this knowledge. In addition, we review the current progress of mass cytometry and high-dimensional cytometric analysis, including the methodology, panel design, experimental procedure, and choice of computational algorithms with a special focus on their utility in exploration of human T cell immunology.
质谱流式细胞术和高维单细胞数据分析的进展将细胞免疫学研究带入了一个新时代。通过将这两个强大的技术平台相结合，免疫学家现在有了更多的工具来解析免疫细胞亚群的巨大多样性及其异质性功能。自从首次引入质谱流式细胞术以来，已经有许多使用这项新技术研究各种细胞类型的报告发表。最初，使用质谱流式细胞术研究人类造血干细胞和外周血 CD8(+)T 细胞的研究揭示了未来研究免疫细胞表型和功能多样性的实验方法。在这里，我们简要回顾一下过去和现在对 T 细胞异质性的理解，以及促进这些知识的技术。此外，我们还回顾了质谱流式细胞术和高维细胞计量分析的最新进展，包括方法学、面板设计、实验程序以及计算算法的选择，特别关注它们在探索人类 T 细胞免疫学中的应用。
