Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Elgomhoria Street 60, 35516, Mansoura, Egypt.
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, D-35392, Gießen, Germany.
BMC Vet Res. 2020 Aug 14;16(1):292. doi: 10.1186/s12917-020-02508-y.
The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius).
The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level.
Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species.
本研究旨在对从奥卡皮鹿（Okapia johnstoni）和王蛇（Python regius）中分离出的两株化脓隐秘杆菌（Trueperella pyogenes）进行表型和基因型特征分析。
通过表型特征、基质辅助激光解吸电离飞行时间质谱分析（MALDI-TOF MS）和先前开发的环介导等温扩增（LAMP）检测 T. pyogenes 伴侣蛋白编码基因 cpn60，可确认物种身份。此外，16S 核糖体 RNA（rRNA）基因、16S-23S rDNA 间隔区（ISR）、编码细菌 RNA 聚合酶β亚基的 rpoB 基因、编码延伸因子 tu 的 tuf 基因和编码潜在毒力因子 pyolysin 的 plo 基因的测序，可将两种 T. pyogenes 分离株鉴定到种的水平。
两种菌株均可明确鉴定为 T. pyogenes。从奥卡皮鹿阴道分泌物中大量分离的 T. pyogenes 菌株似乎对感染过程很重要；而从王蛇中分离出的 T. pyogenes 菌株可从非传染性过程中分离出来。然而，这两种菌株均代表了从这些动物物种中首次分离到 T. pyogenes。
